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dc.contributor.authorRADOMSKI, MAREKen
dc.contributor.authorMEDINA MARTIN, CARLOSen
dc.date.accessioned2011-12-15T12:15:54Z
dc.date.available2011-12-15T12:15:54Z
dc.date.issued2011en
dc.date.submitted2011en
dc.identifier.citationCorbalan, J.J., Medina, C., Jacoby, A., Malinski, T., Radomski, M.W., Amorphous silica nanoparticles trigger nitric oxide/peroxynitrite imbalance in human endothelial cells inflammatory and cytotoxicity effects., International Journal of Nanomedicine, 6, 2011, 1 - 15en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/61288
dc.descriptionPUBLISHEDen
dc.description.abstractBACKGROUND: The purpose of this study was to investigate the mechanism of noxious effects of amorphous silica nanoparticles on human endothelial cells. METHODS: Nanoparticle uptake was examined by transmission electron microscopy. Electrochemical nanosensors were used to measure the nitric oxide (NO) and peroxynitrite (ONOO(-)) released by a single cell upon nanoparticle stimulation. The downstream inflammatory effects were measured by an enzyme-linked immunosorbent assay, real-time quantitative polymerase chain reaction, and flow cytometry, and cytotoxicity was measured by lactate dehydrogenase assay. RESULTS: We found that the silica nanoparticles penetrated the plasma membrane and rapidly stimulated release of cytoprotective NO and, to a greater extent, production of cytotoxic ONOO(-). The low [NO]/[ONOO(-)] ratio indicated increased nitroxidative/oxidative stress and correlated closely with endothelial inflammation and necrosis. This imbalance was associated with nuclear factor ?B activation, upregulation of key inflammatory factors, and cell death. These effects were observed in a nanoparticle size-dependent and concentration-dependent manner. CONCLUSION: The [NO]/[ONOO(-)] imbalance induced by amorphous silica nanoparticles indicates a potentially deleterious effect of silica nanoparticles on vascular endothelium.en
dc.description.sponsorshipThis project was funded by a Science Foundation Ireland grant to MWR and a Trinity College Dublin award to JJC. The authors would like to thank Lorraine O?Driscoll, Lidia Tajber, and Neal Leddy for their technical assistance. CM is a Science Foundation Ireland Stokes Lecturer.en
dc.format.extent1en
dc.format.extent15en
dc.language.isoenen
dc.relation.ispartofseriesInternational Journal of Nanomedicineen
dc.relation.ispartofseries6en
dc.rightsYen
dc.subjectPharmacologyen
dc.subjectnanotoxicologyen
dc.subjectamorphous silica nanoparticlesen
dc.titleAmorphous silica nanoparticles trigger nitric oxide/peroxynitrite imbalance in human endothelial cells inflammatory and cytotoxicity effects.en
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/radomskmen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/martinc2en
dc.identifier.rssinternalid75551en
dc.identifier.rssurihttp://dx.doi.org/10.2147/IJN.S25071en


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