dc.contributor.author | SMYTH, PAUL | en |
dc.contributor.author | O'LEARY, JOHN | en |
dc.contributor.author | O'TOOLE, SHARON | en |
dc.contributor.author | SPILLANE, CATHY | en |
dc.contributor.author | MARTIN, CARA | en |
dc.contributor.author | GALLAGHER, MICHAEL | en |
dc.contributor.author | NORRIS, LUCY | en |
dc.contributor.author | CONLON, NIAMH | en |
dc.contributor.author | MCEVOY, LYNDA MARIE | en |
dc.contributor.author | FFRENCH, GEORGE BRENDAN | en |
dc.contributor.author | STORDAL, BRITTA | en |
dc.contributor.author | KEEGAN, HELEN | en |
dc.contributor.author | FINN, STEPHEN | en |
dc.contributor.author | MCENEANEY, VICTORIA | en |
dc.contributor.author | SHEILS, ORLA | en |
dc.contributor.author | CROWLEY, DARRAGH | en |
dc.date.accessioned | 2011-10-20T11:24:38Z | |
dc.date.available | 2011-10-20T11:24:38Z | |
dc.date.issued | 2011 | en |
dc.date.submitted | 2011 | en |
dc.identifier.citation | Karl Egan., Darragh Crowley., Paul Smyth, Sharon O'Toole, Cathy Spillane, Cara Martin, Michael Gallagher, Aoife Canney, Lucy Norris, Niamh Conlon, Lynda McEvoy2, Brendan Ffrench2, Britta Stordal, Helen Keegan, Stephen Finn, Victoria McEneaney, Alex Laios, Jens Ducre, Eimear Dunne, Leila Smith, Michael Berndt, Orla Sheils, Dermot Kenny, John O'Leary, Platelet Adhesion and Degranulation Induce Pro-Survival and Pro-Angiogenic Signalling in Ovarian Cancer Cells, PLoS ONE, 6, 10, e26125, 2011 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/60228 | |
dc.description | PUBLISHED | en |
dc.description.abstract | Thrombosis is common in ovarian cancer. However, the interaction of platelets with ovarian cancer cells has not been
critically examined. To address this, we investigated platelet interactions in a range of ovarian cancer cell lines with different
metastatic potentials [HIO-80, 59M, SK-OV-3, A2780, A2780cis]. Platelets adhered to ovarian cancer cells with the most
significant adhesion to the 59M cell line. Ovarian cancer cells induced platelet activation [P-selectin expression] in a dose
dependent manner, with the most significant activation seen in response to the 59M cell line. The platelet antagonists
[cangrelor, MRS2179, and apyrase] inhibited 59M cell induced activation suggesting a P2Y12 and P2Y1 receptor mediated
mechanism of platelet activation dependent on the release of ADP by 59M cells. A2780 and 59M cells potentiated PAR-1,
PAR-4, and TxA2 receptor mediated platelet activation, but had no effect on ADP, epinephrine, or collagen induced
activation. Analysis of gene expression changes in ovarian cancer cells following treatment with washed platelets or platelet
releasate showed a subtle but valid upregulation of anti-apoptotic, anti-autophagy pro-angiogenic, pro-cell cycle and
metabolic genes. Thus, ovarian cancer cells with different metastatic potential adhere and activate platelets differentially
while both platelets and platelet releasate mediate pro-survival and pro-angiogenic signals in ovarian cancer cells. | en |
dc.description.sponsorship | This research was supported by a grant from Science Foundation Ireland [SFI] as part of the Biomedical Diagnostics Institute [BDI-2 Centre for Science
Excellence and Technology (CSET)]. KE is supported through the National Biophotonics and Imaging Platform, Ireland, and funded by the Irish Government?s
Programme for Research in Third Level Institutions, Cycle 4, National Development Plan 2007-2013. DC is supported by the Health Research Board (Ireland)
Scholars Programme: Molecular Medicine - From Genes to Function. BF and LMcE are supported by the Emer Casey Foundation, Ireland. The funders had no role
in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | PLoS ONE | en |
dc.relation.ispartofseries | 6 | en |
dc.relation.ispartofseries | 10, e26125 | en |
dc.rights | Y | en |
dc.subject | Oncology | en |
dc.subject | Ovarian cancer | en |
dc.subject | Thrombosis | en |
dc.title | Platelet Adhesion and Degranulation Induce Pro-Survival and Pro-Angiogenic Signalling in Ovarian Cancer Cells | en |
dc.type | Journal Article | en |
dc.contributor.sponsor | Health Research Board (HRB) | en |
dc.contributor.sponsor | Higher Education Authority (HEA) | en |
dc.contributor.sponsor | Science Foundation Ireland (SFI) | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/smythpa | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/shotoole | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/finns | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/gallagmi | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/osheils | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/lnorris | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/mceneanv | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/cspilla | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/cmartin3 | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/olearyjj | en |
dc.identifier.rssinternalid | 75470 | en |
dc.subject.TCDTheme | Cancer | en |
dc.subject.TCDTheme | Genes & Society | en |
dc.identifier.rssuri | http://dx.doi.org/10.1371/journal.pone.0026125 | en |