Hands-free sample preparation platform for nucleic acid analysis.
O'LEARY, JOHN JAMES
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Citation:Baier T, Hansen-Hagge TE, Gransee R, Crombe A, Schmahl S, Paulus C, Drese KS, Keegan H, Martin C, O'Leary JJ, Furuberg L, Solli L, Gr?nn P, Falang IM, Karlg?rd A, Gulliksen A, Karlsen F, Hands-free sample preparation platform for nucleic acid analysis., Lab on a chip, 9, 23, 2009, 3399-405
A Lab-On-Chip system with an instrument is presented which is capable of performing total sample preparation and automated extraction of nucleic acid from human cell samples fixed in a methanol based solution. The target application is extraction of mRNA from cervical liquid based cytology specimens for detection of transformed HPV-infections. The device accepts 3 ml of sample and performs the extraction in a disposable polymer chip of credit card size. All necessary reagents for cell lysis, washing, and elution are stored on-chip and the extraction is performed in two filter stages; one for cell pre-concentration and the other for nucleic acid capture. Tests performed using cancer cell lines and cervical liquid based cytology specimens confirm the extraction of HPV-mRNA by the system.
Keywords:Medical laboratory technology
Publisher:Cambridge University Press
Series/Report no:Lab on a chip;