dc.contributor.author | KELLEHER, DERMOT P | |
dc.date.accessioned | 2009-10-23T17:59:56Z | |
dc.date.available | 2009-10-23T17:59:56Z | |
dc.date.issued | 1995 | |
dc.date.submitted | 1995 | en |
dc.identifier.citation | M. A. O'Connell, R. Cleere, A. Long, L. A. O'Neill and D. Kelleher `Cellular proliferation and activation of NF kappa B are induced by autocrine production of tumor necrosis factor alpha in the human T lymphoma line HuT 78? in The Journal of Biological Chemistry, 270, (13), 1995, pp 7399-7404 | en |
dc.identifier.other | Y | en |
dc.identifier.other | Y | |
dc.identifier.other | 21356 | |
dc.identifier.uri | http://hdl.handle.net/2262/34208 | |
dc.description | PUBLISHED | en |
dc.description.abstract | Tumor necrosis factor (TNF) is a pleiotropic cytokine which has both cytotoxic and proliferative effects. HuT 78, a T-cell line derived from a Sezary lymphoma, is resistant to the cytotoxic effects of TNF, suggesting that TNF may be a growth factor for this cell line. The aim of this study was to determine whether autocrine TNF production could function as a growth factor for HuT 78. Resting HuT 78 and K-4 cells, a protein kinase C-beta-deficient clone of HuT 78, both produced significant amounts of TNF compared with Jurkat cells. Thymidine incorporation by HuT 78 and K-4 cells was inhibited by 90.5 and 73.2%, respectively, with addition of a neutralizing monoclonal antibody to TNF alpha, suggesting that TNF is an autocrine growth factor for these cells. HuT 78 and K-4 cells also expressed high levels of constitutively active NF kappa B, unlike Jurkat cells, which expressed high levels only upon activation with TNF or phorbol 12-myristate 13-acetate. p50 was the major component in the NF kappa B complexes in HuT 78 and K-4 cells. Anti-TNF alpha antibody dramatically decreased levels of NF kappa B in both HuT 78 and K-4 cells. As the TNF gene has an NF kappa B binding motif, an autocrine loop involving TNF induction of NF kappa B is therefore likely in these cells. These findings in a neoplastic T-cell line suggest that therapy directed against TNF could be effective in a subset of T-cell lymphomas. | en |
dc.description.sponsorship | This work was supported by grants from the Cancer Research Advancement Board of the Irish Cancer Society and by the Health Research Board. | en |
dc.format.extent | 7399-7404 | en |
dc.format.extent | 5723465 bytes | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | en |
dc.publisher | The American Society for Biochemistry and Molecular Biology | en |
dc.relation.ispartofseries | The Journal of Biological Chemistry | en |
dc.relation.ispartofseries | 270 | en |
dc.relation.ispartofseries | 13 | en |
dc.rights | Y | en |
dc.subject | Clinical Medicine | en |
dc.title | Cellular proliferation and activation of NF kappa B are induced by autocrine production of tumor necrosis factor alpha in the human T lymphoma line HuT 78 | en |
dc.type | Journal Article | en |
dc.contributor.sponsor | Health Research Board | |
dc.contributor.sponsor | Irish Cancer Society | |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/kellehdp | |
dc.identifier.rssinternalid | 20842 | |