Browsing Zoology (Scholarly Publications) by Author "Murphy, Paula"
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3D representation of Wnt and Frizzled gene expression patterns in the mouse embryo at embryonic day 11.5 (Ts 19).
Murphy, Paula (2008)Wnt signalling is one of the fundamental cell communication systems operating in the embryo and the collection of 19 Wnt and 10 Frizzled (Fzd) receptor genes (in mouse and human) represent just part of a complex system ... -
Abnormal Notochord Branching is Associated with Foregut Abnormalities in an Adriamycin Treated Mouse Model
Murphy, Paula (University of Edinburgh (datashare), 2011)Oesophageal atresia (OA) and tracheooesophageal fistula (TOF) are relatively common human congenital malformations of the foregut where the oesophagus does not connect with the stomach and there is an abnormal connection ... -
Abnormal notochord branching is associated with foregut malformations in the adriamycin treated mouse model.
Murphy, Paula (University of Edinburgh (datashare), 2011)Oesophageal atresia (OA) and tracheooesophageal fistula (TOF) are relatively common human congenital malformations of the foregut where the oesophagus does not connect with the stomach and there is an abnormal connection ... -
Biophysical stimuli induced by passive movements compensate for lack of skeletal muscle during embryonic skeletogenesis
Prendergast, Patrick; Murphy, Paula (University of Edinburgh (datashare), 2011)In genetically modified mice with abnormal skeletal muscle development, bones and joints are differentially affected by the lack of skeletal muscle.We hypothesise that unequal levels of biophysical stimuli in the ... -
Developing bones are differentially affected by compromised skeletal muscle formation
Prendergast, Patrick; Murphy, Paula (University of Edinburgh (datashare), 2010)Mechanical forces are essential for normal adult bone function and repair, but the impact of prenatal muscle contractions on bone development remains to be explored in depth in mammalian model systems. In this study, we ... -
Effects of abnormal muscle forces on prenatal joint morphogenesis in mice
Rolfe, Rebecca; Murphy, Paula (2019)Fetal movements are essential for normal development of the human skeleton. When fetal movements are reduced or restricted, infants are at higher risk of developmental dysplasia of the hip and arthrogryposis (multiple joint ... -
Gene expression analysis of canonical Wnt pathway transcriptional regulators during early morphogenesis of the facial region in the mouse embryo
Murphy, Paula (University of Edinburgh (datashare), 2009)Structures and features of the face, throat and neck are formed from a series of branchial arches that grow out along the ventrolateral aspect of the embryonic head. Multiple signaling pathways have been implicated in ... -
Geometric analysis of chondrogenic self-organisation of embryonic limb bud cells in micromass culture.
Murphy, Paula; Rolfe, Rebecca (Springer Nature, 2022)Spatial and temporal control of chondrogenesis generates precise, species-specific patterns of skeletal structures in the developing vertebrate limb. The pattern-template is laid down when mesenchymal cells at the core ... -
Identification of Mechanosensitive Genes during Embryonic Bone Formation.
Murphy, Paula; Prendergast, Patrick; Nowlan, Niamh (2008)Although it is known that mechanical forces are needed for normal bone development, the current understanding of how biophysical stimuli are interpreted by and integrated with genetic regulatory mechanisms is limited. ... -
Identification of mechanosensitive genes during skeletal development: alteration of genes associated with cytoskeletal rearrangement and cell signalling pathways.
Murphy, Paula; Kelly, Daniel; Kenny, Elaine (University of Edinburgh (datashare), 2014) -
The importance of foetal movement for co-ordinated cartilage and bone development in utero : clinical consequences and potential for therapy.
Murphy, Paula (University of Edinburgh (datashare), 2015) -
Integrated analysis of Wnt signalling system component gene expression
Murphy, Paula (2022)Wnt signalling controls patterning and differentiation across many tissues and organs of the developing embryo through temporally and spatially restricted expression of multi-gene families encoding ligands, receptors, ... -
Investigating the mechanistic basis of biomechanical input controlling skeletal development: exploring the interplay with Wnt signalling at the joint
Rolfe, Rebecca; Murphy, Paula (University of Edinburgh (datashare), 2018)Embryo movement is essential to the formation of a functional skeleton. Using mouse and chick models, we previously showed that mechanical forces influence gene regulation and tissue patterning, particularly at developing ... -
Joint development recovery on resumption of embryonic movement following paralysis
Murphy, Paula; Rolfe, Rebecca (2021)Fetal activity in utero is a normal part of pregnancy and reduced or absent movement can lead to long-term skeletal defects, such as Fetal Akinesia Deformation Sequence, joint dysplasia and arthrogryposis. A variety of ... -
Localization of YAP activity in developing skeletal rudiments is responsive to mechanical stimulation
Murphy, Paula; Rolfe, Rebecca (University of Edinburgh (datashare), 2020)Background: Normal skeletal development, in particular ossification, joint formation and shape features of condyles, depends on appropriate mechanical input from embryonic movement but it is unknown how such physical stimuli ... -
Mechanical Regulation of Skeletal Development
Murphy, Paula; Rolfe, Rebecca (University of Edinburgh (datashare), 2013)Development of the various components of a normal skeleton requires highly regulated signalling systems that co-ordinate spatial and temporal patterns of cell division, cell differentiation and morphogenesis. Much work in ... -
Mechanical Stimulation via Muscle Activity is Necessary for the Maturation of Tendon Multiscale Mechanics during Embryonic Development
Murphy, Paula; Rolfe, Rebecca (2021)During embryonic development, tendons transform into a hypocellular tissue with robust tensile load-bearing capabilities. Previous work suggests that this mechanical transformation is due to increases in collagen fibril ... -
Transcriptome analysis of the mouse E14.5 (TS23) developing humerus and differential expression in muscle-less mutant embryos lacking mechanical stimulation
Murphy, Paula; Rolfe, Rebecca (University of Edinburgh (datashare), 2014)Mechanical stimulation is important for the correct formation of the skeleton. Splotch-delayed mutant embryos (Pax3Spd/Spd) that develop with no limb muscle and therefore no limb movement experience an altered mechanical ... -
WlzWarp: An Open-Source Tool for Complex Alignment of Spatial Data
Murphy, Paula (2022)Background: The spatio-temporal organisation of many biological processes such as gene-expression and neuronal activity is critical to understanding the overall biological behaviour, phenotype and disease. This is ...