Tup1-Ssn6 and Swi-Snf remodelling activities influence long-range chromatin organization upstream of the yeast SUC2 gene.
Citation:
Alastair Fleming and Sari Pennings, Tup1-Ssn6 and Swi-Snf remodelling activities influence long-range chromatin organization upstream of the yeast SUC2 gene., Nucleic Acids Research, 35, 16, 2007, 5520 - 5531Download Item:
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Abstract:
The traditional model for chromatin remodelling
during transcription has focused upon the remodelling
of nucleosomes at gene promoters. However, in
this study, we have determined that Tup1-Ssn6 and
Swi-Snf chromatin remodelling activities extend far
upstream of the SUC2 gene promoter into the
intergenic region of the Saccharomyces cerevisiae
chromosome. We mapped the nucleosomal array
over a 7.5 kb region that encompassed the SUC2
gene promoter and upstream region but was devoid
of other transcriptionally active genes. Nucleosome
positioning over this region was determined under
conditions of glucose repression and derepression,
and in snf2, ssn6 and snf2 ssn6 mutant strains.
A map detailing remodelling events extending as
much as 5 kb upstream of the SUC2 gene promoter
underlines the roles of the Tup1-Ssn6 and Swi-Snf
complexes in respectively organizing and disrupting
nucleosome arrays. The gene specificity of these
events suggests a role in gene regulation. We
propose that long-range chromatin remodelling
activities of Swi-Snf and Tup1-Ssn6 may ultimately
influence whether the chromosomal state of the
SUC2 gene is proficient for transcription. These data
raise the possibility that remodelling of extensive
chromatin domains may be a general property of the
complexes.
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http://people.tcd.ie/fleminalDescription:
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Author: FLEMING, ALASTAIR
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Oxford University PressType of material:
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Nucleic Acids Research;35, 16Availability:
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