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Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/64037

Title: The Development of Non-Viral Gene-Activated Matrices for Bone Regeneration Using Polyethyleneimine (PEI) and Collagen-Based Scaffolds
Author: O'BRIEN, FERGAL
DUFFY, GARRY
Sponsor: 
Name Grant Number
239685
2007-2013
04/Yl1/B531

Author's Homepage: http://people.tcd.ie/fobrien
http://people.tcd.ie/gaduffy
Keywords: Bioengineering
scaffolds
Issue Date: 2012
Citation: Erica G. Tierney, Garry P. Duffy, Alan J. Hibbitts, Sally-Ann Cryan, Fergal J. O'Brien, The Development of Non-Viral Gene-Activated Matrices for Bone Regeneration Using Polyethyleneimine (PEI) and Collagen-Based Scaffolds, Journal of Controlled Release, 2012
Series/Report no.: Journal of Controlled Release
Abstract: The healing potential of scaffolds for tissue engineering can be enhanced by combining them with genes to produce gene-activated matrices (GAMs) for tissue regeneration. We examined the potential of using polyethyleneimine (PEI) as a vector for transfection of mesenchymal stem cells (MSCs) in monolayer culture and in 3D collagen-based GAMs. PEI-pDNA polyplexes were fabricated at a range of N/P ratios and their optimal transfection parameters (N/P 7 ratio, 2 μg dose) and transfection efficiencies (45 ± 3%) determined in monolayer culture. The polyplexes were then loaded onto collagen, collagen-glycosaminoglycan and collagen-nanohydroxyapatite scaffolds where gene expression was observed up to 21 days with a polyplex dose as low as 2 μg. Transient expression profiles indicated that the GAMs act as a polyplex depot system whereby infiltrating cells become transfected over time as they migrate throughout the scaffold. The collagen-nHa GAM exhibited the most prolonged and elevated levels of transgene expression. This research has thus demonstrated that PEI is a highly efficient pDNA transfection agent for both MSC monolayer cultures and in the 3D GAM environment. By combining therapeutic gene therapy with highly engineered scaffolds, it is proposed that these GAMs might have immense capability to promote tissue regeneration.
Description: IN_PRESS
URI: http://hdl.handle.net/2262/64037
Related links: http://dx.doi.org/10.1016/j.jconrel.2011.11.026
Appears in Collections:Administrative Staff Authors (Scholarly Publications)

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The Development of Non-Viral Gene-Activated Matrices for Bone Regeneration Using Polyethyleneimine (PEI) and Collagen-Based Scaffolds.pdfPublished (author's copy) - Peer Reviewed874.4 kBAdobe PDFView/Open
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