The University of Dublin | Trinity College -- Ollscoil Átha Cliath | Coláiste na Tríonóide
Trinity's Access to Research Archive
Home :: Log In :: Submit :: Alerts ::

School of Dental Sciences >
Dental Science >
Dental Science (Scholarly Publications) >

Please use this identifier to cite or link to this item:

Title: Base-metal dental casting alloy biocompatibility assessment using a human-derived 3D oral mucosal model
Author's Homepage:
Keywords: Dentistry
Nickel-chromium (Ni-Cr)
Biocompatibility assessment
Issue Date: 2012
Citation: McGinley, E.L., Moran, G.P., Fleming, G.J.P., Base-metal dental casting alloy biocompatibility assessment using a human-derived 3D oral mucosal model, Acta Biomaterialia, 8, 1, 2012, 432 - 438
Series/Report no.: Acta Biomaterialia
Abstract: Nickel-chromium (Ni-Cr) alloys used in fixed prosthodontics have been associated with type IV nickel-induced hypersensitivity. We hypothesized the full-thickness human-derived oral mucosa model employed for biocompatibility testing of base-metal dental alloys would provide insights into mechanisms of nickel-induced toxicity. Primary oral keratinocytes and gingival fibroblasts were seeded onto Allodermâ„¢ and maintained until full-thickness was achieved prior to Ni-Cr and cobalt-chromium (Co-Cr) alloy-disc exposure (2-72 h). Biocompatibility assessment involved histological analyses with cell viability measurements, oxidative stress responses, inflammatory cytokine expression and cellular toxicity analyses. Inductively coupled plasma mass spectrometry (ICP-MS) analysis determined elemental ion release levels. We detected adverse morphology with significant reductions in cell viability, significant increases in oxidative stress, inflammatory cytokine expression and cellular toxicity for the Ni-Cr alloy-treated oral mucosal models compared with untreated oral mucosal models and adverse effects were increased for the Ni-Cr alloy that leached the most nickel. Co-Cr demonstrated significantly enhanced biocompatibility compared with Ni-Cr alloy-treated oral mucosal models. The human-derived full-thickness oral mucosal model was discriminatory between dental alloys and mechanistically provided insights into Ni-induced toxicity, highlighting potential clinical relevance.
Description: PUBLISHED
Related links:
Appears in Collections:Dental Science (Scholarly Publications)

Files in This Item:

File Description SizeFormat
Base-metal dental casting alloy biocompatibility assessment using a human-derived 3D oral mucosal model.pdfPublished (author's copy) - Peer Reviewed870.01 kBAdobe PDFView/Open

This item is protected by original copyright

Please note: There is a known bug in some browsers that causes an error when a user tries to view large pdf file within the browser window. If you receive the message "The file is damaged and could not be repaired", please try one of the solutions linked below based on the browser you are using.

Items in TARA are protected by copyright, with all rights reserved, unless otherwise indicated.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback