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Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/61635

Title: Base-metal dental casting alloy biocompatibility assessment using a human-derived 3D oral mucosal model
Author: MORAN, GARY
MC GINLEY, EMMA LOUISE
FLEMING, GARRY
Author's Homepage: http://people.tcd.ie/flemingg
http://people.tcd.ie/gmoran
http://people.tcd.ie/emcginle
Keywords: Dentistry
Nickel-chromium (Ni-Cr)
alloys
Biocompatibility assessment
Issue Date: 2012
Citation: McGinley, E.L., Moran, G.P., Fleming, G.J.P., Base-metal dental casting alloy biocompatibility assessment using a human-derived 3D oral mucosal model, Acta Biomaterialia, 8, 1, 2012, 432 - 438
Series/Report no.: Acta Biomaterialia
8
1
Abstract: Nickel-chromium (Ni-Cr) alloys used in fixed prosthodontics have been associated with type IV nickel-induced hypersensitivity. We hypothesized the full-thickness human-derived oral mucosa model employed for biocompatibility testing of base-metal dental alloys would provide insights into mechanisms of nickel-induced toxicity. Primary oral keratinocytes and gingival fibroblasts were seeded onto Allodermâ„¢ and maintained until full-thickness was achieved prior to Ni-Cr and cobalt-chromium (Co-Cr) alloy-disc exposure (2-72 h). Biocompatibility assessment involved histological analyses with cell viability measurements, oxidative stress responses, inflammatory cytokine expression and cellular toxicity analyses. Inductively coupled plasma mass spectrometry (ICP-MS) analysis determined elemental ion release levels. We detected adverse morphology with significant reductions in cell viability, significant increases in oxidative stress, inflammatory cytokine expression and cellular toxicity for the Ni-Cr alloy-treated oral mucosal models compared with untreated oral mucosal models and adverse effects were increased for the Ni-Cr alloy that leached the most nickel. Co-Cr demonstrated significantly enhanced biocompatibility compared with Ni-Cr alloy-treated oral mucosal models. The human-derived full-thickness oral mucosal model was discriminatory between dental alloys and mechanistically provided insights into Ni-induced toxicity, highlighting potential clinical relevance.
Description: PUBLISHED
URI: http://hdl.handle.net/2262/61635
Related links: http://dx.doi.org/10.1016/j.actbio.2011.08.017
Appears in Collections:Dental Science (Scholarly Publications)

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