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Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/60228

Title: Platelet Adhesion and Degranulation Induce Pro-Survival and Pro-Angiogenic Signalling in Ovarian Cancer Cells
Author: STORDAL, BRITTA KRISTINA
O'TOOLE, SHARON
MC ENEANEY, VICTORIA
SPILLANE, CATHY
KEEGAN, HELEN
SMYTH, PAUL
MARTIN, CARA
O'LEARY, JOHN JAMES
CONLON, NIAMH
FINN, STEPHEN
SHEILS, ORLA
GALLAGHER, MICHAEL
NORRIS, LUCY ANGELA
CROWLEY, DARRAGH
FFRENCH, GEORGE BRENDAN
MCEVOY, LYNDA MARIE
Sponsor: Health Research Board
Science Foundation Ireland
Higher Education Authority
Author's Homepage: http://people.tcd.ie/crowleda
http://people.tcd.ie/stordalb
http://people.tcd.ie/shotoole
http://people.tcd.ie/mceneanv
http://people.tcd.ie/cspilla
http://people.tcd.ie/keeganhe
http://people.tcd.ie/smythpa
http://people.tcd.ie/cmartin3
http://people.tcd.ie/olearyjj
http://people.tcd.ie/conlonn3
http://people.tcd.ie/finns
http://people.tcd.ie/osheils
http://people.tcd.ie/gallagmi
http://people.tcd.ie/lnorris
http://people.tcd.ie/crowleda
http://people.tcd.ie/ffrenchg
http://people.tcd.ie/lmmcevoy
Keywords: Oncology
Ovarian cancer
Thrombosis
Issue Date: 2011
Publisher: PLoS
Citation: Karl Egan., Darragh Crowley., Paul Smyth, Sharon O Toole, Cathy Spillane, Cara Martin, Michael Gallagher, Aoife Canney, Lucy Norris, Niamh Conlon, Lynda McEvoy2, Brendan Ffrench2, Britta Stordal, Helen Keegan, Stephen Finn, Victoria McEneaney, Alex Laios, Jens Ducre, Eimear Dunne, Leila Smith, Michael Berndt, Orla Sheils, Dermot Kenny, John O Leary, Platelet Adhesion and Degranulation Induce Pro-Survival and Pro-Angiogenic Signalling in Ovarian Cancer Cells, PLoS ONE, 6, 10, e26125, 2011
Series/Report no.: PLoS ONE;
6;
10, e26125;
Abstract: Thrombosis is common in ovarian cancer. However, the interaction of platelets with ovarian cancer cells has not been critically examined. To address this, we investigated platelet interactions in a range of ovarian cancer cell lines with different metastatic potentials [HIO-80, 59M, SK-OV-3, A2780, A2780cis]. Platelets adhered to ovarian cancer cells with the most significant adhesion to the 59M cell line. Ovarian cancer cells induced platelet activation [P-selectin expression] in a dose dependent manner, with the most significant activation seen in response to the 59M cell line. The platelet antagonists [cangrelor, MRS2179, and apyrase] inhibited 59M cell induced activation suggesting a P2Y12 and P2Y1 receptor mediated mechanism of platelet activation dependent on the release of ADP by 59M cells. A2780 and 59M cells potentiated PAR-1, PAR-4, and TxA2 receptor mediated platelet activation, but had no effect on ADP, epinephrine, or collagen induced activation. Analysis of gene expression changes in ovarian cancer cells following treatment with washed platelets or platelet releasate showed a subtle but valid upregulation of anti-apoptotic, anti-autophagy pro-angiogenic, pro-cell cycle and metabolic genes. Thus, ovarian cancer cells with different metastatic potential adhere and activate platelets differentially while both platelets and platelet releasate mediate pro-survival and pro-angiogenic signals in ovarian cancer cells.
Description: PUBLISHED
URI: http://hdl.handle.net/2262/60228
Related links: http://dx.doi.org/10.1371/journal.pone.0026125
Appears in Collections:Clinical Medicine (Scholarly Publications)

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