The University of Dublin | Trinity College -- Ollscoil Átha Cliath | Coláiste na Tríonóide
Trinity's Access to Research Archive
Home :: Log In :: Submit :: Alerts ::

TARA >
Administrative Staff Authors  >
Administrative Staff Authors (Scholarly Publications) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/57288

Title: Analysis of dynamic tyrosine phosphoproteome in LFA-1 triggered migrating T-cells.
Author: FREELEY, MICHAEL GERARD
VERMA, NAVIN KUMAR
VOLKOV, YURI
KELLEHER, DERMOT P
DEMPSEY, EUGENE
LONG, AIDEEN
Sponsor: Health Research Board
Higher Education Authority
Author's Homepage: http://people.tcd.ie/longai
http://people.tcd.ie/freeleym
http://people.tcd.ie/vermank
http://people.tcd.ie/yvolkov
http://people.tcd.ie/kellehdp
http://people.tcd.ie/dempseeu
Keywords: Immunology
T-lymphocytes
Issue Date: 2011
Citation: Verma NK, Dempsey E, Freeley M, Botting CH, Long A, Kelleher D, Volkov Y, Analysis of dynamic tyrosine phosphoproteome in LFA-1 triggered migrating T-cells., Journal of Cellular Physiology, 226, 6, 2011, 1489 1498
Series/Report no.: Journal of Cellular Physiology
226
6
Abstract: The ordered, directional migration of T-lymphocytes is a key process during immune surveillance and response. This requires cell adhesion to the high endothelial venules or to the extracellular matrix by a series of surface receptor/ligand interactions involving adhesion molecules of the integrin family including lymphocyte function associated molecule-1 (LFA-1) and intercellular adhesion molecules (ICAMs). Reversible protein phosphorylation is emerging as a key player in the regulation of biological functions with tyrosine phosphorylation playing a crucial role in signal transduction. Thus, the study of this type of post-translational modification at the proteomic level has great biological significance. In this work, phospho-enriched cell lysates from LFA-1-triggered migrating human T-cells were subjected to immunoaffinity purification of tyrosine phosphorylated proteins, mass spectrometric, and bioinformatic analysis. In addition to the identification of several well-documented proteins, the analysis suggested involvement of a number of new and novel proteins in LFA-1 induced T-cell migration. This dataset expands the list of the signaling components of the LFA-1 induced phosphotyrosine protein complexes in migrating T-cells that will be extremely useful in the study of their specific roles within LFA-1 associated signaling pathways. Identification of proteins previously not reported in the context of LFA-1 stimulated signal transduction might provide new insights into understanding the LFA-1 signaling networks and aid in the search for new potential therapeutic targets.
Description: PUBLISHED
URI: http://hdl.handle.net/2262/57288
Related links: http://dx.doi.org/10.1002/jcp.22478
Appears in Collections:Administrative Staff Authors (Scholarly Publications)

Files in This Item:

File Description SizeFormat
Analysis of Dynamic Tyrosine Phosphoproteome in LFA-1 Triggered Migrating T-Cells.pdfPublished (author's copy) - Peer Reviewed594.67 kBAdobe PDFView/Open


This item is protected by original copyright


Please note: There is a known bug in some browsers that causes an error when a user tries to view large pdf file within the browser window. If you receive the message "The file is damaged and could not be repaired", please try one of the solutions linked below based on the browser you are using.

Items in TARA are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback