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Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/55775

Title: CHARACTERIZATION OF A NOVEL ARGININE CATABOLIC MOBILE ELEMENT (ACME) AND STAPHYLOCOCCAL CHROMOSOMAL CASSETTE mec COMPOSITE ISLAND WITH SIGNIFICANT HOMOLOGY TO STAPHYLOCOCCUS EPIDERMIDIS ACME TYPE II IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS GENOTYPE ST22-MRSA-IV
Author: COLEMAN, DAVID
Sponsor: 
Name Grant Number
TRA/2006/4

Author's Homepage: http://people.tcd.ie/dcoleman
Keywords: ACME
ST22-MRSA-IV
SCCmec
ST8-MRSA-IVa/USA300
DNA microarray
Issue Date: 2011
Citation: SHORE, A.C., ROSSNEY, A.S., BRENNAN, O.M., KINNEVEY, P., HUMPHREYS, H., SULLIVAN, D.J., GOERING, R.V., EHRICHT, R., MONECKE, S., COLEMAN, D.C., CHARACTERIZATION OF A NOVEL ARGININE CATABOLIC MOBILE ELEMENT (ACME) AND STAPHYLOCOCCAL CHROMOSOMAL CASSETTE mec COMPOSITE ISLAND WITH SIGNIFICANT HOMOLOGY TO STAPHYLOCOCCUS EPIDERMIDIS ACME TYPE II IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS GENOTYPE ST22-MRSA-IV, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 55, 5, 2011, 1896 - 1905
Series/Report no.: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
55
5
Abstract: The arginine catabolic mobile element (ACME) is prevalent among ST8-MRSA-IVa (USA300) isolates and evidence suggests that ACME enhances the ability of ST8-MRSA-IVa to grow and survive on its host. ACME has been identified in a small number of isolates belonging to other MRSA clones but is widespread among coagulase-negative staphylococci (CoNS). This study reports the first description of ACME in two distinct strains of the pandemic ST22-MRSA-IV clone. A total of 238 MRSA isolates recovered in Ireland between 1971 and 2008 were investigated for ACME using a DNA microarray. Twenty-three isolates (9.7%) were ACME-positive, all were either MRSA genotype ST8-MRSA-IVa (7/23, 30%) or ST22-MRSA-IV (16/23, 70%). Whole-genome sequencing and comprehensive molecular characterization revealed the presence of a novel 46-kb ACME and SCCmec composite island (ACME/SCCmec-CI) in ST22-MRSA-IVh isolates (n = 15). This ACME/SCCmec-CI consists of a 12-kb DNA region previously identified in ACME type II in S. epidermidis ATCC 12228, a truncated copy of the J1 region of SCCmec I and a complete SCCmec IVh element. The composite island has a novel genetic organization with ACME located within orfX and SCCmec located downstream of ACME. One pvl-positive ST22-MRSA-IVa isolate carried ACME located downstream of SCCmec IVa as previously described in ST8-MRSA-IVa. These results suggest that ACME has been acquired by ST22-MRSA-IV on two independent occasions. At least one of these instances may have involved horizontal transfer and recombination events between MRSA and CoNS. The presence of ACME may enhance dissemination of ST22-MRSA-IV, an already successful MRSA clone.
Description: PUBLISHED
22 February, Epub ahead of print
URI: http://hdl.handle.net/2262/55775
Related links: http://dx.doi.org/10.1128/AAC.01756-10
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=21343442&dopt=Abstract
Appears in Collections:Dental Science (Scholarly Publications)

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