CzrB is a putative zinc transporter from Thermus thermophilus. The protein is
proposed to consist of a hexahelical transmembrane domain with a cytosolic
extramembranal C-terminus. The latter 92-residue fragment may be expressed
free and may function independently of the full-length integral membrane
protein. A 6 His-tagged form of the water-soluble fragment has been
overexpressed in Escherichia coli and diffraction-quality crystals of the tagged
and tag-free variants have been grown. Preliminary X-ray analyses of tag-free
fragment crystals with (2.2 A ° resolution) and without zinc ions (1.7 A °
resolution) reveal that the former has at least two zinc ions bound per monomer.
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