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Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/50461

Title: Crystallization and preliminary X-ray diffraction analysis of a soluble domain of the putative zinc transporter CzrB from Thermus thermophilus.
Author: CAFFREY, MARTIN
Sponsor: Science Foundation Ireland
Author's Homepage: http://people.tcd.ie/mcaffre
Keywords: Biophysics
Thermus thermophilus
diffraction analysis
Issue Date: 2007
Publisher: International Union of Crystallography
Citation: Höfer, N., Kolaj, O., Li, H., Cherezov, V., Gillilan, R., Wall, J., Caffrey, M., Crystallization and preliminary X-ray diffraction analysis of a soluble domain of the putative zinc transporter CzrB from Thermus thermophilus., Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 63, 8, 2007, 673-677
Series/Report no.: Acta Crystallographica Section F: Structural Biology and Crystallization Communications;
63;
8;
Abstract: CzrB is a putative zinc transporter from Thermus thermophilus. The protein is proposed to consist of a hexahelical transmembrane domain with a cytosolic extramembranal C-terminus. The latter 92-residue fragment may be expressed free and may function independently of the full-length integral membrane protein. A 6 His-tagged form of the water-soluble fragment has been overexpressed in Escherichia coli and diffraction-quality crystals of the tagged and tag-free variants have been grown. Preliminary X-ray analyses of tag-free fragment crystals with (2.2 A ° resolution) and without zinc ions (1.7 A ° resolution) reveal that the former has at least two zinc ions bound per monomer.
Description: PUBLISHED
URI: http://hdl.handle.net/2262/50461
Related links: http://dx.doi.org/10.1107/S1744309107032277
Appears in Collections:Biochemistry (Scholarly Publications)

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