The University of Dublin | Trinity College -- Ollscoil Átha Cliath | Coláiste na Tríonóide
Trinity's Access to Research Archive
Home :: Log In :: Submit :: Alerts ::

TARA >
School of Biochemistry & Immunology >
Biochemistry >
Biochemistry (Scholarly Publications) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/49865

Title: Crystallizing Transmembrane Peptides in Lipidic Mesophases
Author: CAFFREY, MARTIN
Sponsor: 
Name Grant Number
GM75915
CM0902
07/IN.1/B1836

Author's Homepage: http://people.tcd.ie/mcaffre
Keywords: Biochemistry
Peptides
membrane proteins
Issue Date: 2010
Citation: Höfer N, Aragão D, Caffrey M, Crystallizing Transmembrane Peptides in Lipidic Mesophases, Biophysical Journal, 99, 3, 2010, L23-L25
Series/Report no.: Biophysical Journal
99
3
Abstract: Structure determination of membrane proteins by crystallographic means has been facilitated by crystallization in lipidic mesophases. It has been suggested, however, that this so-called in meso method, as originally implemented, would not apply to small protein targets having ≤4 transmembrane crossings. In our study, the hypothesis that the inherent flexibility of the mesophase would enable crystallogenesis of small proteins was tested using a transmembrane pentadecapeptide, linear gramicidin, which produced structure-grade crystals. This result suggests that the in meso method should be considered as a viable means for high-resolution structure determination of integral membrane peptides, many of which are predicted to be coded for in the human genome.
Description: PUBLISHED
URI: http://hdl.handle.net/2262/49865
Related links: http://dx.doi.org/10.1016/j.bpj.2010.05.011
Access: OpenAccess
Appears in Collections:Biochemistry (Scholarly Publications)

Files in This Item:

File Description SizeFormat
Crystallizing Transmembrane Peptides in Lipidic Mesophases - supplemental.pdfPublished (author's copy) - Peer Reviewed538.69 kBAdobe PDFView/Open
Crystallizing Transmembrane Peptides in Lipidic Mesophases.pdfPublished (author's copy) - Peer Reviewed189.8 kBAdobe PDFView/Open


This item is protected by original copyright


Please note: There is a known bug in some browsers that causes an error when a user tries to view large pdf file within the browser window. If you receive the message "The file is damaged and could not be repaired", please try one of the solutions linked below based on the browser you are using.

Items in TARA are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback