The University of Dublin | Trinity College -- Ollscoil Átha Cliath | Coláiste na Tríonóide
Trinity's Access to Research Archive
Home :: Log In :: Submit :: Alerts ::

TARA >
School of Pharmacy and Pharmaceutical Sciences >
Pharmacy and Pharmaceutical Sciences >
Pharmacy and Pharmaceutical Sciences (Scholarly Publications) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/41267

Title: A role for adenosine A1 receptor blockade in the ability of caffeine to promote MDMA "Ecstasy"-induced striatal dopamine release
Author: HARKIN, ANDREW
Sponsor: Health Research Board
Author's Homepage: http://people.tcd.ie/aharkin
Keywords: Neuropharmacology
MDMA
Issue Date: 2011
Citation: Natacha Vanattou-Saïfoudine, Anna Gossen, Andrew Harkin, A role for adenosine A1 receptor blockade in the ability of caffeine to promote MDMA "Ecstasy"-induced striatal dopamine release, European Journal of Pharmacology, 650, 1, 2011, 220-228
Series/Report no.: European Journal of Pharmacology
650
1
Abstract: Co-administration of caffeine profoundly enhances the acute toxicity of 3,4 methylenedioxymethamphetamine (MDMA) in rats. The aim of this study was to determine the ability of caffeine to impact upon MDMA-induced dopamine release in superfused brain tissue slices as a contributing factor to this drug interaction. MDMA (100 and 300 μM) induced a dose-dependent increase in dopamine release in striatal and hypothalamic tissue slices preloaded with [3 H] dopamine (1 μM). Caffeine (100 μM) also induced dopamine release in the striatum and hypothalamus, albeit to a much lesser extent than MDMA. When striatal tissue slices were superfused with MDMA (30 μM) in combination with caffeine (30 μM), caffeine enhanced MDMA-induced dopamine release, provoking a greater response than that obtained following either caffeine or MDMA applications alone. The synergistic effects in the striatum were not observed in hypothalamic slices. As adenosine A1 receptors, one of the main pharmacological targets of caffeine, are known to play an important role in the regulation of dopamine release, their role in the modulation of MDMA-induced dopamine release was investigated. 1 μM 8-cyclopentyl-1,3-dipropylxanthine (DPCPX), a specific A1 antagonist, like caffeine, enhanced MDMA-induced dopamine release from striatal slices while 1 μM 2,chloro-N(6)-cyclopentyladenosine (CCPA), a selective adenosine A1 receptor agonist, attenuated this. Treatment with either SCH 58261, a selective A2A receptor antagonist, or rolipram, a selective PDE-4 inhibitor, failed to reproduce a caffeine-like effect on MDMA-induced dopamine release. These results suggest that caffeine regulates MDMA-induced dopamine release in striatal tissue slices, via inhibition of adenosine A1 receptors.
Description: PUBLISHED
URI: http://hdl.handle.net/2262/41267
Related links: http://dx.doi.org.elib.tcd.ie/10.1016/j.ejphar.2010.10.012
Appears in Collections:Pharmacy and Pharmaceutical Sciences (Scholarly Publications)

Files in This Item:

File Description SizeFormat
A role for adenosine A1 receptor blockade in the ability of caffeine to promote MDMA “Ecstasy”-induced striatal dopamine release .pdfPublished (author's copy) - Peer Reviewed504.27 kBAdobe PDFView/Open


This item is protected by original copyright


Please note: There is a known bug in some browsers that causes an error when a user tries to view large pdf file within the browser window. If you receive the message "The file is damaged and could not be repaired", please try one of the solutions linked below based on the browser you are using.

Items in TARA are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback