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Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/41227

Title: IDENTIFICATION AND CHARACTERIZATION OF THE MULTIDRUG RESISTANCE GENE cfr IN PANTON-VALENTINE LEUKOCIDIN-POSITIVE ST8-METHICILLIN-RESISTANT Staphylococcus aureus-IVa (USA300)
Author: SHORE, ANNA
COLEMAN, DAVID
BRENNAN, ORLA
Sponsor: 
Name Grant Number
TRA/2006/4

Author's Homepage: http://people.tcd.ie/dcoleman
http://people.tcd.ie/ashore
http://people.tcd.ie/brennaor
Keywords: Immunology
Staphylococcus aureus
Issue Date: 2010
Citation: SHORE AC, BRENNAN OM, EHRICHT R, MONECKE S, SCHWARZ S, SLICKERS P, COLEMAN DC, IDENTIFICATION AND CHARACTERIZATION OF THE MULTIDRUG RESISTANCE GENE cfr IN PANTON-VALENTINE LEUKOCIDIN-POSITIVE ST8-METHICILLIN-RESISTANT Staphylococcus aureus-IVa (USA300), ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 54, 12, 2010, 4978 - 4984
Series/Report no.: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
54
12
Abstract: The staphylococcal cfr gene mediates resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A, a phenotype that has been termed PhLOPSA. The cfr gene has mainly been associated with coagulase-negative staphylococcal isolates from animals, and only a few cfr-positive methicillin-resistant Staphylococcus aureus (MRSA) isolates have been described so far. This study reports the first description of a cfr-positive MRSA isolate (M05/0060) belonging to the pandemic Panton-Valentine leukocidin (PVL)-positive sequence type 8 MRSA IVa/USA300 (ST8-MRSA-IVa/USA300) clone. The cfr gene was detected in M05/0060 using a DNA microarray which was used to screen PVL-positive MRSA isolates for the presence of virulence genes, typing markers, and antimicrobial resistance genes. Antimicrobial susceptibility testing revealed that M05/0060 exhibited the cfr-associated resistance phenotype. Molecular analysis identified the presence of cfr and a second phenicol resistance gene, fexA, on a novel 45-kb conjugative plasmid, which was designated pSCFS7. Within pSCFS7, a DNA segment consisting of cfr, a truncated copy of insertion sequence IS21-558, and a region with homology to the DNA invertase gene bin3 of transposon Tn552 from Bacillus mycoides was integrated into the transposase gene tnpB of the fexA-carrying transposon Tn558. The emergence of a multidrug-resistant cfr-positive variant of ST8-MRSA-IVa/USA300 is alarming and requires ongoing surveillance. Moreover, the identification of a novel conjugative plasmid carrying the cfr gene indicates the ability of cfr to spread to other MRSA strains.
Description: PUBLISHED
4 October; Epub ahead of print
URI: http://hdl.handle.net/2262/41227
Related links: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=20921317&dopt=Abstract
Appears in Collections:Dental Science (Scholarly Publications)

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