The University of Dublin | Trinity College -- Ollscoil Átha Cliath | Coláiste na Tríonóide
Trinity's Access to Research Archive
Home :: Log In :: Submit :: Alerts ::

TARA >
School of Engineering >
Mechanical & Manufacturing Eng >
Mechanical & Manufacturing Eng (Scholarly Publications) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/41118

Title: Engineering of large cartilaginous constructs through the use of microchanneled hydrogels and rotational culture
Author: BUCKLEY, CONOR TIMOTHY
KELLY, DANIEL
Sponsor: Science Foundation Ireland
Author's Homepage: http://people.tcd.ie/kellyd9
http://people.tcd.ie/cbuckle
Keywords: Bioengineering
Cartilage repair
Issue Date: 2009
Publisher: Mary Ann Liebert
Citation: Buckley, C.T., Thorpe, S.D., Kelly, D.J., Engineering of large cartilaginous constructs through the use of microchanneled hydrogels and rotational culture, Tissue Engineering A, 15, 11, 2009, 3213-3220
Series/Report no.: Tissue Engineering A;
15;
11;
Abstract: The development of functional engineered cartilaginous tissues of sufficient size that can be used clinically to treat large defects remains a major and significant challenge. This study investigated if the introduction of microchannels into chondrocyte-seeded agarose hydrogels would result in the formation of a superior and more homogenous cartilaginous tissue due to enhanced nutrient transport. Microchannel construct cylinders were fabricated via a moulding process utilising a pillared structure to create the required architecture. Constructs were subjected to either constant rotation in a rotational bioreactor system or free swelling conditions. After 28 days of free swelling culture the presence of microchannels did not enhance GAG accumulation within the core of the construct compared to solid constructs (0.317 ± 0.002 % w/w vs. 0.401 ± 0.020 % w/w). However under dynamically rotating conditions, GAG accumulation in the cores (1.165 ± 0.132 % w/w) of microchannel constructs were similar to that in the periphery (1.23 ± 0.074 % w/w) of solid constructs, although still significantly lower than their corresponding periphery (1.64 ± 0.133 % w/w) after 28 days. These results confirm that cellular nutrient consumption is primarily responsible for creating the spatial gradients in molecules regulating the biosynthetic activity of chondrocytes through the volume of hydrogels, and that changing the scaffold architecture alone may have little effect while the inherent diffusivity of the material remains high. Rather a combination of forced convection and modified scaffold architecture is necessary to engineer large cartilaginous tissues in vitro.
Description: PUBLISHED
URI: http://hdl.handle.net/2262/41118
Appears in Collections:Mechanical & Manufacturing Eng (Scholarly Publications)

Files in This Item:

File Description SizeFormat
engineering.pdfAccepted for publication (author's copy) - Peer Reviewed568.66 kBAdobe PDFView/Open


This item is protected by original copyright


Please note: There is a known bug in some browsers that causes an error when a user tries to view large pdf file within the browser window. If you receive the message "The file is damaged and could not be repaired", please try one of the solutions linked below based on the browser you are using.

Items in TARA are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback