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Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/38817

Title: Functional Properties of Cartilaginous Tissues Engineered From Infrapatellar Fat Pad Derived Mesenchymal Stem Cells.
Author: VINARDELL, TATIANA
BUCKLEY, CONOR TIMOTHY
KELLY, DANIEL
Sponsor: Science Foundation Ireland
Author's Homepage: http://people.tcd.ie/kellyd9
http://people.tcd.ie/vinardt
http://people.tcd.ie/cbuckle
Keywords: Cartilage repair
agarose hydrogel
Mesenchymal Stem Cells
infrapatellar fat pad
functional tissue engineering
Issue Date: 2010
Citation: Buckley, C T; Vinardell, T; Thorpe, S D; Jones, E; McGonagle, D; Kelly, D J., Functional Properties of Cartilaginous Tissues Engineered From Infrapatellar Fat Pad Derived Mesenchymal Stem Cells., Journal of Biomechanics, 43, 5, 2010, 920-926
Series/Report no.: Journal of Biomechanics
43
5
Abstract: Articular cartilage has a poor intrinsic capacity for self-repair. The advent of autologous chondrocyte implantation has provided a feasible method to treat cartilage defects. However, the associated drawbacks with the isolation and expansion of chondrocytes from autologous tissue has prompted research into alternative cell sources such as mesenchymal stem cells (MSCs) which have been found to exist in the bone marrow as well as other joint tissues such as the infrapatellar fat pad (IFP), synovium and within the synovial fluid itself. In this work we assessed the chondrogenic potential of IFP-derived porcine cells over a 6 week period in agarose hydrogel culture in terms of mechanical properties, biochemical content and histology. It was found that IFP cells underwent robust chondrogenesis as assessed by glycosaminoglycan (1.47+/-0.22% w/w) and collagen (1.44+/-0.22% w/w) accumulation after 42 days of culture. The 1Hz dynamic modulus of the engineered tissue at this time point was 272.8kPa (+/-46.8). The removal of TGF-beta3 from culture after 21 days was shown to have a significant effect on both the mechanical properties and biochemical content of IFP constructs after 42 days, with minimal increases occurring from day 21 to day 42 without continued supplementation of TGF-beta3. These findings further strengthen the case that the IFP may be a promising cell source for putative cartilage repair strategies.
Description: PUBLISHED
PMID: 20005518
URI: http://hdl.handle.net/2262/38817
Related links: http://dx.doi.org/10.1016/j.jbiomech.2009.11.005
Appears in Collections:Mechanical & Manufacturing Eng (Scholarly Publications)

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