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Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/36710

Title: Pivotal Advance: Avian colony-stimulating factor 1 (CSF-1), interleukin-34 (IL-34), and CSF-1 receptor genes and gene products
Author: FARES, MARIO ALI
Author's Homepage: http://people.tcd.ie/faresm
Keywords: Chicken
zebra finch
macrophage
co-evolution
Issue Date: 2010
Citation: Garceau V, Smith J, Paton IR, Davey M, Fares MA, Sester DP, Burt DW, Hume DA., Pivotal Advance: Avian colony-stimulating factor 1 (CSF-1), interleukin-34 (IL-34), and CSF-1 receptor genes and gene products, Journal of Leukocyte Biology, 87, 2010, 753-764
Series/Report no.: Journal of Leukocyte Biology
87
Abstract: Macrophages are involved in many aspects of development, host defense, pathology, and homeostasis. Their normal differentiation, proliferation, and survival are controlled by CSF-1 via the activation of the CSF-1R. A recently discovered cytokine, IL-34, was shown to bind the same receptor in humans. Chicken is a widely used model organism in developmental biology, but the factors that control avian myelopoiesis have not been identified previously. The CSF-1, IL-34, and CSF1R genes in chicken and zebra finch were identified from respective genomic/cDNA sequence resources. Comparative analysis of the avian CSF1R loci revealed likely orthologs of mammalian macrophage-specific promoters and enhancers, and the CSF1R gene is expressed in the developing chick embryo in a pattern consistent with macrophage-specific expression. Chicken CSF-1 and IL-34 were expressed in HEK293 cells and shown to elicit macrophage growth from chicken BM cells in culture. Comparative sequence and co-evolution analysis across all vertebrates suggests that the two ligands interact with distinct regions of the CSF1R. These studies demonstrate that there are two separate ligands for a functional CSF1R across all vertebrates.
Description: PUBLISHED
URI: http://dx.doi.org/10.1189/jlb.0909624
http://hdl.handle.net/2262/36710
Related links: http://dx.doi.org/10.1189/jlb.0909624
Appears in Collections:Genetics (Scholarly Publications)

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