The University of Dublin | Trinity College -- Ollscoil Átha Cliath | Coláiste na Tríonóide
Trinity's Access to Research Archive
Home :: Log In :: Submit :: Alerts ::

TARA >
School of Physics >
Physics >
Physics (Scholarly Publications) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/36707

Title: Energy transfer processes in semiconductor quantum dots: bacteriorhodopsin hybrid system
Author: RAKOVICH, YURY
DONEGAN, JOHN FRANCIS
Sponsor: Irish Research Council for Science Engineering and Technology
Author's Homepage: http://people.tcd.ie/jdonegan
http://people.tcd.ie/rakovicy
Keywords: quantum dots
semiconductor nanocrystals
bacteriorhodopsin
purple membranes
energy transfer
FRET
luminescence quenching
hybrid materials
Issue Date: 2009
Publisher: SPIE
Citation: Aliaksandra Rakovich, Alyona Sukhanova, Nicolas Bouchonville, Michael Molinari, Michel Troyon, Jacques H. M. Cohen, Yury Rakovich, John F. Donegan, and Igor Nabiev, Energy transfer processes in semiconductor quantum dots: bacteriorhodopsin hybrid system, Proceedings of SPIE, Photonic Materials, Devices, and Applications III, Dresden, Germany, 4 May 2009, Ali Serpenguzel, 736620, 2009
Series/Report no.: 736620
Abstract: The potential impact of nanoscience on energy transfer processes in biomolecules was investigated on the example of a complex between fluorescent semiconductor nanocrystals and photochromic membrane protein. The interactions between colloidal CdTe quantum dots (QDs) and bacteriorhodopsin (bR) protein were studied by a variety of spectroscopic techniques, including integrated and time-resolved fluorescence spectroscopies, zeta potential and size measurement, and fluorescence correlation spectroscopy. QDs’ luminescence was found to be strongly modulated by bacteriorhodopsin, but in a controllable way. Decreasing emission lifetimes and blue shifts in QDs’ emission at increasing protein concentrations suggest that quenching occurs via Förster resonance energy transfer. On the other hand, concave Stern-Volmer plots and sigmoidal photoluminescence quenching curves imply that the self-assembling of NCs and bR exists, and the number of nanocrystals (NCs) per bacteriorhodopsin contributing to energy transfer can be determined from the inflection points of sigmoidal curves. This number was found to be highly dependent not only on the spectral overlap between NC emission and bR absorption bands, but also on nanocrystal surface charge. These results demonstrate the potential of how inorganic nanoscale materials can be employed to improve the generic molecular functions of biomolecules. The observed interactions between CdTe nanocrystals and bacteriorhodopsin can provide the basis for the development of novel functional materials with unique photonic properties and applications in areas such as all-optical switching, photovoltaics and data storage.
Description: PUBLISHED
URI: http://dx.doi.org/10.1117/12.821731
http://hdl.handle.net/2262/36707
Appears in Collections:Physics (Scholarly Publications)

Files in This Item:

File Description SizeFormat
Energy transfer processes.pdfPublished (publisher's copy) - Peer Reviewed2.03 MBAdobe PDFView/Open


This item is protected by original copyright


Please note: There is a known bug in some browsers that causes an error when a user tries to view large pdf file within the browser window. If you receive the message "The file is damaged and could not be repaired", please try one of the solutions linked below based on the browser you are using.

Items in TARA are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback