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Title: A differential role for actin in the life cycle of T. brucei.
Sponsor: Wellcome Trust
Author's Homepage:
Keywords: actin
Trypanosoma brucei
Issue Date: 2004
Publisher: Nature Publishing Group
Citation: García-Salcedo J.A., Perez-Morga, D., Gijón P., Dilbeek, V., Pays, E. and Nolan, D.P., A differential role for actin in the life cycle of T. brucei., EMBO Journal, 25, 2004, 780, 789
Series/Report no.: EMBO Journal
Abstract: Actin is expressed at similar levels but in different locations in bloodstream and procyclic forms of Trypanosoma brucei. In bloodstream forms actin colocalizes with the highly polarized endocytic pathway, whereas in procyclic forms it is distributed throughout the cell. RNA interference demonstrated that in bloodstream forms, actin is an essential protein. Depletion of actin resulted in a rapid arrest of cell division, termination of vesicular traffic from the flagellar pocket membrane leading to gross enlargement of the pocket, loss of endocytic activity and eventually cell death. These results indicate that actin is required for the formation of coated vesicles from the flagellar pocket membrane, which is the first step in the endocytic pathway. Although loss of actin in procyclic cells did not affect growth, the trans region of the Golgi became distorted and enlarged and appeared to give rise to a heterogeneous population of vesicles. However, the flagellar pocket was not affected. These findings suggest that trypanosomes have different functional requirements for actin during the bloodstream and procyclic phases of the life cycle.
Description: PUBLISHED
Appears in Collections:Biochemistry (Scholarly Publications)

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