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Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/35146

Title: Developing bones are differentially affected by compromised skeletal muscle formation
Author: PRENDERGAST, PATRICK JOHN
MURPHY, PAULA
Sponsor: Wellcome Trust
Irish Research Council for Science Engineering and Technology
Author's Homepage: http://people.tcd.ie/pmurphy3
http://people.tcd.ie/pprender
Keywords: Mechanobiology; mechanical forces; embryonic bone formation; muscle contractions; endochondral ossification
Issue Date: 2010
Citation: Niamh C. Nowlan, Céline Bourdon, Shahragim Tajbakhsh, Gérard Dumas, Patrick J. Prendergast and Paula Murphy, Developing bones are differentially affected by compromised skeletal muscle formation, Bone, 46, 2010, 1275 1285
Series/Report no.: Bone
46
Abstract: Mechanical forces are essential for normal adult bone function and repair, but the impact of prenatal muscle contractions on bone development remains to be explored in depth in mammalian model systems. In this study, we analyse skeletogenesis in two ‘muscleless’ mouse mutant models in which the formation of skeletal muscle development is disrupted; Myf5nlacZ/nlacZ:MyoD-/- and Pax3Sp/Sp (Splotch). Ossification centres were found to be differentially affected in the muscleless limbs, with significant decreases in bone formation in the scapula, humerus, ulna and femur, but not in the tibia. In the scapula and humerus, the morphologies of ossification centres were abnormal in muscleless limbs. Histology of the humerus revealed a decreased extent of the hypertrophic zone in mutant limbs but no change in shape of this region. The elbow joint was also found to be clearly affected with a dramatic reduction in the joint line, while no abnormalities were evident in the knee. The humeral deltoid tuberosity was significantly reduced in size in the Myf5nlacZ/nlacZ:MyoD-/- mutants while a change in shape but not in size was found in the humeral tuberosities of the Pax3Sp/Sp mutants. We also examined skeletal development in a ‘reduced muscle’ model, the Myf5nlacZ/+:MyoD-/- mutant, in which skeletal muscle forms but with reduced muscle mass. The reduced muscle phenotype appeared to have an intermediate effect on skeletal development, with reduced bone formation in the scapula and humerus compared to controls, but not in other rudiments. In summary, we have demonstrated that skeletal development is differentially affected by the lack of skeletal muscle, with certain rudiments and joints being more severely affected than others. These findings indicate that the response of skeletal progenitor cells to biophysical stimuli may depend upon their location in the embryonic limb, implying a complex interaction between mechanical forces and location-specific regulatory factors affecting bone and joint development.
Description: PUBLISHED
URI: http://dx.doi.org/10.1016/j.bone.2009.11.026
http://hdl.handle.net/2262/35146
Appears in Collections:Zoology (Scholarly Publications)

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