The University of Dublin | Trinity College -- Ollscoil Átha Cliath | Coláiste na Tríonóide
Trinity's Access to Research Archive
Home :: Log In :: Submit :: Alerts ::

School of Natural Science >
Zoology >
Zoology (Scholarly Publications) >

Please use this identifier to cite or link to this item:

Title: Effects of retinoic acid excess on expression oh Hox 2.9 and Krox 20 and on morphological segmentation in the hindbrain of mouse embryos
Author's Homepage:
Keywords: Zoology
Issue Date: 1991
Publisher: Nature Publishing Group
Citation: Morriss-Kay, G.M., Murphy, P., Hill, R.E. and Davidson, D.R. ‘Effects of retinoic acid excess on expression oh Hox 2.9 and Krox 20 and on morphological segmentation in the hindbrain of mouse embryos’ in EMBO Journal, 10, 1991, pp 2985 - 2995
Series/Report no.: EMBO Journal
Abstract: Mouse embryos were exposed to maternally administered RA on day 8.0 or day 7 3/4 of development, i.e. at or just before the differentiation of the cranial neural plate, and before the start of segmentation. On day 9.0, the RA-treated embryos had a shorter preotic hindbrain than the controls and clear rhombomeric segmentation was absent. These morphological effects were correlated with alterations in the spatiotemporal distribution patterns of two genes, Hox-2.9 and Krox-20, which are expressed in the otic and preotic hindbrain and in specific neural crest cell populations. Hox-2.9 was expressed throughout the preotic hindbrain region, instead of being confined to rhombomere 4. Krox-20 was not expressed rostral to the Hox-2.9 domain, i.e. its normal rhombomere 3 domain was absent. The Hox-2.9/Krox-20 boundary was ill-defined, with patches of alternating expression of the two genes. In migrating neural crest cells, Hox-2.9 expression was both abnormally extensive and abnormally prolonged. Neural crest cells expressing Krox-20 remained close to the neural tube. Embryos exposed to RA on day 8 1/4 appeared to be morphologically normal. We suggest that early events leading to rhombomeric segmentation and rhombomere-specific gene expression are specifically vulnerable to raised RA levels, and may require RA levels lower than those in the region of somitic segmentation.
Description: PUBLISHED
Appears in Collections:Zoology (Scholarly Publications)

Files in This Item:

File Description SizeFormat
Morriss-Kay et al.pdfpublished (publisher copy) peer-reviewed3.84 MBAdobe PDFView/Open

This item is protected by original copyright

Please note: There is a known bug in some browsers that causes an error when a user tries to view large pdf file within the browser window. If you receive the message "The file is damaged and could not be repaired", please try one of the solutions linked below based on the browser you are using.

Items in TARA are protected by copyright, with all rights reserved, unless otherwise indicated.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback