The University of Dublin | Trinity College -- Ollscoil Átha Cliath | Coláiste na Tríonóide
Trinity's Access to Research Archive
Home :: Log In :: Submit :: Alerts ::

TARA >
School of Biochemistry & Immunology >
Biochemistry >
Biochemistry (Scholarly Publications) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/33442

Title: Trif-related adapter molecule is phosphorylated by protein kinase C epsilon during Toll-like receptor 4 signalling
Author: MCGETTRICK-DILLON, ANNE
O'NEILL, LUKE ANTHONY JOHN
PALSSON, EVA
Sponsor: Health Research Board
Science Foundation Ireland
Author's Homepage: http://people.tcd.ie/laoneill
Keywords: Biochemistry
Issue Date: 2006
Publisher: National Academy of Sciences
Citation: McGettrick, A, Brint EK, Palsson-McDermott, EM, Rowe, DC, Golenbock, DT, Gay NJ, Fitzgerald, KA and O’Neill LA ‘Trif-related adapter molecule is phosphorylated by protein kinase C epsilon during Toll-like receptor 4 signalling’ in Proceedings of the National Academy of Sciences, 103, (24), 2006, pp 9196 - 9201
Series/Report no.: Proceedings of the National Academy of Sciences
103
24
Abstract: PKCε has been shown to play a key role in the effect of the Gram-negative bacterial product LPS; however, the target for PKCε in LPS signaling is unknown. LPS signaling is mediated by Toll-like receptor 4, which uses four adapter proteins, MyD88, MyD88 adapter-like (Mal), Toll/IL-1R domain-containing adapter inducing IFN-β (Trif), and Trif-related adapter molecule (TRAM). Here we show that TRAM is transiently phosphorylated by PKCε on serine-16 in an LPS-dependent manner. Activation of IFN regulatory factor 3 and induction of the chemokine RANTES, which are both TRAM-dependent, were attenuated in PKCε-deficient cells. TRAMS16A is inactive when overexpressed and is attenuated in its ability to reconstitute signaling in TRAM-deficient cells. We have therefore uncovered a key process in Toll-like receptor 4 signaling, identifying TRAM as the target for PKCε.
Description: PUBLISHED
URI: http://dx.doi.org/10.1073/pnas.0600462103
http://hdl.handle.net/2262/33442
Appears in Collections:Biochemistry (Scholarly Publications)

Files in This Item:

File Description SizeFormat
Trif-related adapter molecule is phosphorylated by PKC{epsilon} during Toll-like receptor 4 signaling.pdfpublished (publisher copy) peer-reviewed993.1 kBAdobe PDFView/Open


This item is protected by original copyright


Please note: There is a known bug in some browsers that causes an error when a user tries to view large pdf file within the browser window. If you receive the message "The file is damaged and could not be repaired", please try one of the solutions linked below based on the browser you are using.

Items in TARA are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback