D.J. Kelly, A. Crawford, S.C. Dickinson, T.J. Sims, A.P. Hollander, P.J. Prendergast and P.V. Hatton ‘Biochemical markers of the mechanical quality of engineered hyaline cartilage’ in Journal of Materials Science: Materials in Medicine, 18, (2), 2007, pp 273-281
Journal of Materials Science: Materials in Medicine 18 2
The aim of this study was to determine whether or not biochemical markers can be
used as surrogate measures for the mechanical quality of tissue engineered cartilage.
The biochemical composition of tissue engineered cartilage constructs were altered by
varying either (i) the initial cell seeding density of the scaffold (seeding density
protocol) or (ii) the length of time the engineered tissue was cultured (culture period
protocol). The aggregate or Young’s moduli of the constructs were measured (by
confined or unconfined compression respectively), and compared with the
composition of the extracellular matrix by quantitative measurement of the
glycosaminoglycan (GAG), hydroxyproline, collagen I and collagen II and collagen
cross-links. The aggregate modulus correlated positively with both GAG and collagen
II content, but not with collagen I content. Young’s modulus correlated positively
with GAG, collagen II and collagen I content, and the ratio of mature to immature
cross-links, but not with hydroxyproline content. These results suggested that
hydroxyproline may be an unreliable indicator of mechanical quality of tissue
engineered cartilage, and that a measure of collagen II and GAG content is required to
predict the biomechanical quality of tissue engineered cartilage.
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