The University of Dublin | Trinity College -- Ollscoil Átha Cliath | Coláiste na Tríonóide
Trinity's Access to Research Archive
Home :: Log In :: Submit :: Alerts ::

TARA >
School of Engineering >
Mechanical & Manufacturing Eng >
Mechanical & Manufacturing Eng (Scholarly Publications) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/29107

Title: Deformation simulation of cells seeded on a collagen-GAG scaffold in a flow perfusion bioreactor using a sequential 3D CFD-elastostatics model.
Author: KELLY, DANIEL
Sponsor: Science Foundation Ireland
Author's Homepage: http://people.tcd.ie/kellyd9
Keywords: Mechanical & Manufacturing Engineering
Issue Date: 2009
Citation: Jungreuthmayer, C., Jaasma, M.J., Al-Munajjed, A.A., Zanghellini, J., Kelly, D.J., O'Brien, F.J., Deformation simulation of cells seeded on a collagen-GAG scaffold in a flow perfusion bioreactor using a sequential 3D CFD-elastostatics model., Medical Engineering and Physics, 31, 4, 2009, 420-427
Series/Report no.: Medical Engineering and Physics
31
4
Abstract: Tissue-engineered bone shows promise in meeting the huge demand for bone grafts caused by up to 4 million bone replacement procedures per year, worldwide. State-of-the-art bone tissue engineering strategies use flow perfusion bioreactors to apply biophysical stimuli to cells seeded on scaffolds and to grow tissue suitable for implantation into the patient's body. The aim of this study was to quantify the deformation of cells seeded on a collagen-GAG scaffold which was perfused by culture medium inside a flow perfusion bioreactor. Using a μCT scan of an unseeded collagen-GAG scaffold, a sequential 3D CFD-deformation model was developed. The wall shear stress and the hydrostatic wall pressure acting on the cells were computed through the use of a CFD simulation and fed into a linear elastostatics model in order to calculate the deformation of the cells. The model used numerically seeded cells of two common morphologies where cells are either attached flatly on the scaffold wall or bridging two struts of the scaffold. Our study showed that the displacement of the cells is primarily determined by the cell morphology. Although cells of both attachment profiles were subjected to the same mechanical load, cells bridging two struts experienced a deformation up to 500 times higher than cells only attached to one strut. As the scaffold's pore size determines both the mechanical load and the type of attachment, the design of an optimal scaffold must take into account the interplay of these two features and requires a design process that optimizes both parameters at the same time.
Description: PUBLISHED
PMID: 19109048
URI: http://dx.doi.org/10.1016/j.medengphy.2008.11.003
http://hdl.handle.net/2262/29107
Related links: http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T9K-4V6RNTF-2-R&_cdi=5117&_user=103681&_orig=search&_coverDate=05%2F31%2F2009&_sk=999689995&view=c&wchp=dGLbVtz-zSkWb&md5=6b704f588e0e3c6f41a2bcb4088cfcdc&ie=/sdarticle.pdf
Appears in Collections:Mechanical & Manufacturing Eng (Scholarly Publications)

Files in This Item:

File Description SizeFormat
Deformation simulation of cells seeded.pdfpublished (author copy) peer-reviewed886.94 kBAdobe PDFView/Open


This item is protected by original copyright


Please note: There is a known bug in some browsers that causes an error when a user tries to view large pdf file within the browser window. If you receive the message "The file is damaged and could not be repaired", please try one of the solutions linked below based on the browser you are using.

Items in TARA are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback