Vendrell V., Summerhurst K, Sharpe J, Davidson D, Murphy P, Gene expression analysis of canonical Wnt pathway transcriptional regulators during early morphogenesis of the facial region in the mouse embryo, Gene Expression Patterns, 2009
Gene Expression Patterns
Structures and features of the face, throat and neck are formed from a series of branchial
arches that grow out along the ventrolateral aspect of the embryonic head. Multiple
signaling pathways have been implicated in patterning interactions that lead to species
specific growth and differentiation within the branchial region that sculpt these features.
A direct role for Wnt signaling in particular has been shown. The spatial and temporal
distribution of Wnt pathway components contributes to the operation of the signaling
system. We present the precise distribution of gene expression of canonical Wnt pathway
transcriptional regulators, Tcf1, Lef1, Tcf3, Tcf4 and β-catenin between embryonic day
(E) 9.5 and 11.5. In situ hybridization combined with Optical Projection Tomography
was used to record and compare distribution of transcripts in 3D within the developing
branchial arches. This shows widespread yet very specific expression of the gene set
indicating that all genes contribute to proper patterning of the region. Tcf1 and Lef1 are
more prominent in rostral arches, particularly at later ages, and Tcf3 and Tcf4 are in
general expressed more deeply (medial/endodermal aspect) in the arches than Tcf1 and
Lef1. Comparison with Wnt canonical pathway readout patterns shows that the
relationship between the expression of individual transcription factors and activation of
the pathway is not simple, indicating complexity and flexibility in the signaling system.
Please note: There is a known bug in some browsers that causes an
error when a user tries to view large pdf file within the browser window.
If you receive the message "The file is damaged and could not be
repaired", please try one of the solutions linked below based on the
browser you are using.
Items in TARA are protected by copyright, with all rights reserved, unless otherwise indicated.