Wnt Fzd OPT Mouse embryo 3D expression patterns Cell communication systems
Kristen Summerhurst, Margaret Stark, James Sharpe, Duncan Davidson and Paula Murphy, '3D representation of Wnt and Frizzled gene expression patterns in the mouse embryo at embryonic day 11.5 (Ts 19)' in Gene Expression Patterns, 8, (5), 2008, p331 - 348
Gene Expression Patterns 8 (5) 2008
Wnt signalling is one of the fundamental cell communication systems operating in the embryo and
the collection of 19 Wnt and 10 Frizzled (Fzd) receptor genes (in mouse and human) represent just
part of a complex system to be unravelled. Here we present a spatially comprehensive set of data on
the 3D distribution of Wnt and Fzd gene expression patterns at a carefully selected single stage of
mouse development. Overviews and selected features of the patterns are presented and the full 3D
data set, generated by fully described probes, is available to the research community through the
Edinburgh Mouse Atlas of Gene Expression. In addition to being comprehensive, the data set has
been generated and recorded in a consistent manner to facilitate comparisons between gene
expression patterns with the capacity to generate matching virtual sections from the 3D
representations for specific studies. Expression patterns in the left forelimb were selected for more
detailed comparative description. In addition to confirming the previously published expression of
these genes, our whole embryo and limb bud analyses significantly extend the data in terms of details
of the patterns and the addition of previously undetected sites of expression. Our focussed analysis
of expression domains in the limb, defined by just two gene families, reveals a surprisingly high
degree of spatial complexity and underlines the enormous potential for local cellular interactions that
exist within an emerging structure. This work also highlights the use of OPT to generate detailed
high-quality, spatially complex expression data that is readily comparable between specimens and
can be reviewed and reanalysed as required for specific studies. It represents a core set of data that
will be extended with additional stages of development and through addition of potentially interacting
genes and ultimately other cross-regulatory communication pathways operating in the embryo.
Please note: There is a known bug in some browsers that causes an
error when a user tries to view large pdf file within the browser window.
If you receive the message "The file is damaged and could not be
repaired", please try one of the solutions linked below based on the
browser you are using.
Items in TARA are protected by copyright, with all rights reserved, unless otherwise indicated.