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Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/18839

Title: 3D representation of Wnt and Frizzled gene expression patterns in the mouse embryo at embryonic day 11.5 (Ts 19).
Author: MURPHY, PAULA
Sponsor: Science Foundation Ireland
Author's Homepage: http://people.tcd.ie/pmurphy3
Keywords: Wnt
Fzd
OPT
Mouse embryo
3D expression patterns
Cell communication systems
Issue Date: 2008
Publisher: Elsevier
Citation: Kristen Summerhurst, Margaret Stark, James Sharpe, Duncan Davidson and Paula Murphy, '3D representation of Wnt and Frizzled gene expression patterns in the mouse embryo at embryonic day 11.5 (Ts 19)' in Gene Expression Patterns, 8, (5), 2008, p331 - 348
Series/Report no.: Gene Expression Patterns
8 (5) 2008
Abstract: Wnt signalling is one of the fundamental cell communication systems operating in the embryo and the collection of 19 Wnt and 10 Frizzled (Fzd) receptor genes (in mouse and human) represent just part of a complex system to be unravelled. Here we present a spatially comprehensive set of data on the 3D distribution of Wnt and Fzd gene expression patterns at a carefully selected single stage of mouse development. Overviews and selected features of the patterns are presented and the full 3D data set, generated by fully described probes, is available to the research community through the Edinburgh Mouse Atlas of Gene Expression. In addition to being comprehensive, the data set has been generated and recorded in a consistent manner to facilitate comparisons between gene expression patterns with the capacity to generate matching virtual sections from the 3D representations for specific studies. Expression patterns in the left forelimb were selected for more detailed comparative description. In addition to confirming the previously published expression of these genes, our whole embryo and limb bud analyses significantly extend the data in terms of details of the patterns and the addition of previously undetected sites of expression. Our focussed analysis of expression domains in the limb, defined by just two gene families, reveals a surprisingly high degree of spatial complexity and underlines the enormous potential for local cellular interactions that exist within an emerging structure. This work also highlights the use of OPT to generate detailed high-quality, spatially complex expression data that is readily comparable between specimens and can be reviewed and reanalysed as required for specific studies. It represents a core set of data that will be extended with additional stages of development and through addition of potentially interacting genes and ultimately other cross-regulatory communication pathways operating in the embryo.
Description: PUBLISHED
URI: http://dx.doi.org/10.1016/j.gep.2008.01.007
http://hdl.handle.net/2262/18839
ISSN: 49204
Appears in Collections:Zoology (Scholarly Publications)

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