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Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/16479

Title: A dimer of the Toll-like receptor 4 cytoplasmic domain provides a specific scaffold for the recruitment of signalling adaptor proteins.
Author: O'NEILL, LUKE ANTHONY JOHN
Sponsor: Medical Research Council
Biotechnology and Biological Sciences Research Council
Author's Homepage: http://people.tcd.ie/laoneill
Keywords: Toll-like receptor 4
TLR4
transmembrane receptors
immune system
Issue Date: 2007
Publisher: PLoS
Citation: Nuñez Miguel R, Wong J, Westolll JF, Brooks HJ, O'Neill LA, Gay NJ, Bryant, A dimer of the Toll-like receptor 4 cytoplasmic domain provides a specific scaffold for the recruitment of signalling adaptor proteins., PLoS ONE, 2, (8), 2007, pe788.
Series/Report no.: PLoS ONE
2 (8)
Abstract: The Toll-like receptor 4 (TLR4) is a class I transmembrane receptor expressed on the surface of immune system cells. TLR4 is activated by exposure to lipopolysaccharides derived from the outer membrane of Gram negative bacteria and forms part of the innate immune response in mammals. Like other class 1 receptors, TLR4 is activated by ligand induced dimerization, and recent studies suggest that this causes concerted conformational changes in the receptor leading to self association of the cytoplasmic Toll/Interleukin 1 receptor (TIR) signalling domain. This homodimerization event is proposed to provide a new scaffold that is able to bind downstream signalling adaptor proteins. TLR4 uses two different sets of adaptors; TRAM and TRIF, and Mal and MyD88. These adaptor pairs couple two distinct signalling pathways leading to the activation of interferon response factor 3 (IRF-3) and nuclear factor κB (NFκB) respectively. In this paper we have generated a structural model of the TLR4 TIR dimer and used molecular docking to probe for potential sites of interaction between the receptor homodimer and the adaptor molecules. Remarkably, both the Mal and TRAM adaptors are strongly predicted to bind at two symmetry-related sites at the homodimer interface. This model of TLR4 activation is supported by extensive functional studies involving site directed mutagenesis, inhibition by cell permeable peptides and stable protein phosphorylation of receptor and adaptor TIR domains. Our results also suggest a molecular mechanism for two recent findings, the caspase 1 dependence of Mal signalling and the protective effects conferred by the Mal polymorphism Ser180Leu.
Description: PUBLISHED
URI: http://hdl.handle.net/2262/16479
ISSN: 50640
Appears in Collections:Biochemistry (Scholarly Publications)

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