Show simple item record

dc.contributor.authorCorr, Sineaden
dc.contributor.authorO'Neill, Lukeen
dc.contributor.authorJohnston, Danielen
dc.date.accessioned2021-03-02T12:12:16Z
dc.date.available2021-03-02T12:12:16Z
dc.date.issued2018en
dc.date.submitted2018en
dc.identifier.citationDaniel G.W. Johnston, Christoph A. Thaiss, Raul Cabrera-Rubio, Michelle A. Williams, Paul D. Cotter, Eran Elinav, Luke A.J. O?Neill, Sin?ad C. Corr, Loss of microRNA-21 influences the gut microbiota causing reduced susceptibility in a murine model of colitis., Journal of Crohns and Colitis, 12, 7, 2018, 835-en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/95465
dc.descriptionPUBLISHEDen
dc.description.abstractBackground and aims: microRNAs regulate gene expression and influence the pathogenesis of human diseases. The present study investigated the role of microRNA-21 [miR-21] in the pathogenesis of intestinal inflammation, because miR-21 is highly expressed in inflammatory bowel disease. Inflammatory bowel disease is associated with intestinal barrier dysfunction and an altered gut microbiota. Recent studies have demonstrated that host microRNAs can shape the microbiota. Thus, we determined the influence of miR-21 on the gut microbiota and observed the subsequent impact in a dextran sodium sulphate [DSS]-induced colitis model. Methods: The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration. We carried out 16S rRNA sequencing on WT and miR-21-/- mice to dissect potential differences in the gut microbiota. Results: miR-21-/- mice have reduced susceptibility to DSS-induced colitis compared with WT mice. Co-housing conferred some protection to WT mice, while GF mice colonized with fecal homogenate from miR-21-/- were protected from DSS colitis compared with those colonized with WT homogenate. Further supporting a role for the microbiota in the observed phenotype, the protection afforded by miR-21 depletion was lost when mice were pre-treated with antibiotics. The 16S rRNA sequencing revealed significant differences in the composition of WT and miR-21-/- intestinal microbiota. Conclusions: These findings suggest that miR-21 influences the pathogenesis of intestinal inflammation by causing propagation of a disrupted gut microbiota.en
dc.format.extent835en
dc.language.isoenen
dc.relation.ispartofseriesJournal of Crohns and Colitisen
dc.relation.ispartofseries12en
dc.relation.ispartofseries7en
dc.rightsYen
dc.subjectpathogenesis of human diseasesen
dc.subjectdextran sodium sulphate [DSS]-induced colitis model.en
dc.subjectmicroRNAsen
dc.subject.lcshpathogenesis of human diseasesen
dc.subject.lcshdextran sodium sulphate [DSS]-induced colitis model.en
dc.subject.lcshmicroRNAsen
dc.titleLoss of microRNA-21 influences the gut microbiota causing reduced susceptibility in a murine model of colitis.en
dc.typeJournal Articleen
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/corrscen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/djohnsten
dc.identifier.peoplefinderurlhttp://people.tcd.ie/laoneillen
dc.identifier.rssinternalid177016en
dc.identifier.doihttp://dx.doi.org/10.1093/ecco-jcc/jjy038en
dc.rights.ecaccessrightsopenAccess
dc.contributor.sponsorGrantNumber11/SIRG/B2099en
dc.subject.TCDThemeImmunology, Inflammation & Infectionen
dc.subject.TCDTagmicrobiomeen
dc.identifier.orcid_id0000-0001-9930-5039en
dc.status.accessibleNen


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record