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dc.contributor.authorGIORDANI, SILVIA
dc.contributor.authorMOVIA, DANIA
dc.contributor.authorVOLKOV, YURI
dc.contributor.authorPRINA MELLO, ADRIELE
dc.date.accessioned2020-07-30T16:42:45Z
dc.date.available2020-07-30T16:42:45Z
dc.date.issued2010
dc.date.submitted2010en
dc.identifier.citationMovia, D., Prina-Mello, A., Volkov, Y., Giordani, S, Determination of spiropyran cytotoxicity by high content screening and analysis for safe application in bionanosensing, Chemical Research in Toxicology, 2010, 23, 9, 1459 - 1466en
dc.identifier.otherY
dc.identifier.urihttp://hdl.handle.net/2262/93088
dc.descriptionPUBLISHEDen
dc.description.abstractThe in vitro toxic response of spiropyrans in cellular models has not been previously addressed, despite the fact that such photoswitchable molecules have shown great potential as versatile and tunable components for bionanodevices and imaging agents. In this study, we examine the cytotoxic effects of a spiropyran, namely, 8-methoxy-6-nitro-BIPS (1′,3′-dihydro-1′-ethanol-3′,3′-dimethyl-8-methoxy-6-nitro-spiro(2H-1-benzopyran-2,2′-(2H)-indole) [1], in three cultured cellular models (THP-1, AGS, and A549 cell lines) by High Content Screening and Analysis (HCSA) and by enzyme-linked immunosorbent (ELISA) assays (Interleukin-6 and Tumor Necrosis Factor-alpha). The HCSA results show that low concentrations of 8-methoxy-6-nitro-BIPS (10−6, 10−8, and 10−9 M) do not induce any cytotoxic response after 24 and 72 h exposure time, while at the highest concentrations (10−3and 10−4 M) the exposure time becomes a critical parameter of the toxic response. The cell viability is reduced by 60% for THP-1 cells, 50% for AGS cells, and 40% for A549 cells at a spiropyran concentration of 10−3 M after 24 h incubation, whereas at 72 h, the cell loss increases above 90%. Interestingly, at 10−4 M no significant cytotoxic response is registered after 24 h exposure, where contrarily cytotoxicity is verified after 72 h. Our ELISA results show that consistently with the HCSA analysis a robust inflammatory response is present at 10−3 M after 24 h exposure and at 10−3 M and 10−4 M after 72 h, in all three cell lines investigated.en
dc.description.sponsorshipThis work was supported by Science Foundation Ireland under different schemes (PIYRA 07/Y12/I1052, CSET and SRC), Higher Education Authority (HEA) under the PRTLI-4 programme, the Health Research Board and IRCSET (Postgraduate Research Scholarship to Dania Movia). We are grateful to Dr Despina Bazou for the live imaging, Mr Antoin Douglawi and Mr Manuel Natali for the synthesis of the spiropyran. We also would like to thank Ms Fiona Byrne, Dr 15 Anthony Mitchell, Dr Bashir Mohammed (TCD-HCSA laboratory) for the technical support and the helpful advice and discussions.en
dc.format.extent1459en
dc.format.extent1466en
dc.language.isoenen
dc.relation.ispartofseriesChemical Research in Toxicology;
dc.relation.ispartofseries23;
dc.relation.ispartofseries9;
dc.rightsYen
dc.subjectAGS, human gastric cancer cell line; A549, human adenocarcinomicen
dc.subject.lcshAGS, human gastric cancer cell line; A549, human adenocarcinomicen
dc.titleDetermination of spiropyran cytotoxicity by high content screening and analysis for safe application in bionanosensingen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/giordans
dc.identifier.peoplefinderurlhttp://people.tcd.ie/yvolkov
dc.identifier.peoplefinderurlhttp://people.tcd.ie/prinamea
dc.identifier.peoplefinderurlhttp://people.tcd.ie/dmovia
dc.identifier.rssinternalid69090
dc.identifier.doi10.1021/tx100123g
dc.subject.TCDThemeNanoscience & Materialsen
dc.subject.TCDThemeNext Generation Medical Devicesen
dc.subject.TCDTagMedical Devicesen
dc.identifier.rssurihttp://dx.doi.org/10.1021/tx100123g


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