Characterization of proteins associated with the LFA-1 mediated cytoskeletal / signaling complex in locomotory T lymphocytes
Citation:Basma S. E. D. El Homasany, 'Characterization of proteins associated with the LFA-1 mediated cytoskeletal / signaling complex in locomotory T lymphocytes', [thesis], Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Medicine, 2005, pp 291
El Homasany TCD THESIS 7398 Characterization of.pdf (PDF) 176.1Mb
LFA-1 plays a crucial role in all aspects of T cell locomotion in both immune physiological functions and pathological conditions. T cell locomotion is associated with translocation of protein kinase C isoenzymes β and δ to the MTOC and microtubules. To identify key players in LFA-1 signaling in the locomotory T cells, the in situ immunoprecipitation technique was deployed in this study. This technique is based on single-ligand triggering of T cell locomotion via immobilized motility inducing antibodies to LFA-1, which mimics the effect of natural ligands. A number of protein kinases (PKCε, PKC δ, PKAIIα) as well as key cytoskeletal components (α and γ-tubulin) and the motor protein dynein, all of which play critical roles in the T cell locomotion, have been studied in this work and were found to physically associate with the LFA-1 induced cytoskeletal/signaling complex. Moreover, a potential association between LFA-1 and members of the transcription factor NFκB (IκBα, P65) was also elucidated in this thesis.
Author: El Homasany, Basma S. E. D.
Qualification name:Doctor of Philosophy (Ph.D.)
Publisher:Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Medicine
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Type of material:thesis
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