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dc.contributor.advisorPorter, Richard
dc.contributor.authorCarroll, Audrey
dc.date.accessioned2019-05-14T15:18:17Z
dc.date.available2019-05-14T15:18:17Z
dc.date.issued2004
dc.identifier.citationAudrey Carroll, 'An investigation into the expression and physiological function of mitochondrial uncoupling proteins', [thesis], Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2004, pp 267
dc.identifier.otherTHESIS 7419
dc.identifier.urihttp://hdl.handle.net/2262/86803
dc.description.abstractOur study provides evidence that rat thymus contains a functioning mitochondrial uncoupling protein (UCP 1). Using reverse transcriptase-polymerase chain reaction (RT-PCR), we showed that rat thymus contains UCP 1 mRNA. Antipeptide UCP 1 antibodies, that we show to be specific for UCP 1, detected UCP 1 protein in thymus and thymus cell (i.e. thymocyte) mitochondria. We also show that starvation (48-hour) induced no significant change in the abundance of UCP 1 in thymus and thymocyte mitochondria. Evidence for functional UCP 1 in rat thymus mitochondria was obtained using a radiolabeled binding assay in thymus and BAT mitochondria. UCP 1 in thymus mitochondria binds [3H] GDP with similar binding parameters, Bmax and KD values, as BAT mitochondria. In addition, a large component of the non-phosphorylating oxygen consumption by thymus mitochondria was inhibited by purine nucleotides and activated by nanomolar amounts of fatty-acids, akin to UCP 1 in BAT mitochondria. We also purified UCP 1 from thymus mitochondria by hydroxyapatite chromatography and identified the isolated protein by peptide mass mapping and tandem mass spectrometry using MALDI-TOF and LC-MS/MS, respectively. We conclude that rat thymus contains a functioning UCP 1 protein, which has the capacity to regulate metabolic flux and production and transport of reactive oxygen containing molecules in the thymus.
dc.format1 volume
dc.language.isoen
dc.publisherTrinity College (Dublin, Ireland). School of Biochemistry and Immunology
dc.relation.isversionofhttp://stella.catalogue.tcd.ie/iii/encore/record/C__Rb12388008
dc.subjectBiochemistry, Ph.D.
dc.subjectPh.D. Trinity College Dublin
dc.titleAn investigation into the expression and physiological function of mitochondrial uncoupling proteins
dc.typethesis
dc.type.supercollectionthesis_dissertations
dc.type.supercollectionrefereed_publications
dc.type.qualificationlevelDoctoral
dc.type.qualificationnameDoctor of Philosophy (Ph.D.)
dc.rights.ecaccessrightsopenAccess
dc.format.extentpaginationpp 267
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