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dc.contributor.advisorFoster, Tim
dc.contributor.authorKeane, Fiona Mary
dc.date.accessioned2019-04-30T09:28:40Z
dc.date.available2019-04-30T09:28:40Z
dc.date.issued2007
dc.identifier.citationFiona Mary Keane, 'Molecular characterisation of Region A of FnBPA from Staphylococcus aureus', [thesis], Trinity College (Dublin, Ireland). Department of Microbiology, 2007, pp 338
dc.identifier.otherTHESIS 8380
dc.identifier.urihttp://hdl.handle.net/2262/86391
dc.description.abstractThe surface-expressed fibronectin-binding proteins, FnBPA and FnBPB, of Staphylococcus aureus promote attachment to immobilised fibrinogen and elastin via the N-Terminal A region. The N2N3 subdomains of region A were found to contain the minimum ligand-binding activity. A model of the 3D structure of the N2N3 subdomains of FnBPA was created based on the crystal structure of a similar fibrinogen-binding protein, ClfA. This model allowed C-terminal truncates of rAFnBPA to be constructed. Those lacking the latching peptide and preceding hinge region were defective in binding both fibrinogen and elastin indicating that both ligands bind to FnBPA in a similar manner. Further support for this theory was the inhibition of bacteria expressing FnBPA from binding immobilised fibrinogen and elastin by using a monoclonal anti-rAFnBPA antibody and a peptide comprising the C-terminal residues of they chain of fibrinogen.
dc.format1 volume
dc.language.isoen
dc.publisherTrinity College (Dublin, Ireland). Department of Microbiology
dc.relation.isversionofhttp://stella.catalogue.tcd.ie/iii/encore/record/C__Rb13315256
dc.subjectMicrobiology, Ph.D.
dc.subjectPh.D. Trinity College Dublin
dc.titleMolecular characterisation of Region A of FnBPA from Staphylococcus aureus
dc.typethesis
dc.type.supercollectionthesis_dissertations
dc.type.supercollectionrefereed_publications
dc.type.qualificationlevelDoctoral
dc.type.qualificationnameDoctor of Philosophy (Ph.D.)
dc.rights.ecaccessrightsopenAccess
dc.format.extentpaginationpp 338
dc.description.noteTARA (Trinity's Access to Research Archive) has a robust takedown policy. Please contact us if you have any concerns: rssadmin@tcd.ie


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