Investigations into the physiological expression of UCP1 and UCP3
Citation:Clare M. Brennan, 'Investigations into the physiological expression of UCP1 and UCP3', [thesis], Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2005, pp 204
Brennan TCD THESIS 8007 Investigations into.pdf (PDF) 100.4Mb
Previous studies have indicated that acute exercise induces large increases in uncoupling protein 3 in skeletal muscle, whereas endurance training results in marked decreases in skeletal muscle uncoupling protein 3 at both mRNA and protein levels. Using an uncoupling protein 3 specific antibody we confirm that uncoupling protein 3 protein is expressed in rat skeletal muscle mitochondria. In addition, we show that absolute uncoupling protein 3 expression is elevated in gastrocneumis and plantaris muscle homogenates following acute exercise (2 weeks). Furthermore, we show the novel finding that uncoupling protein 3 protein expression per unit mass of mitochondria is increased by acute exercise (2 weeks) in gastrocnemius muscle of mice. We also show that the increase in uncoupling protein 3 protein expression is transient with levels returning to pre-exercise levels 22 hours after the final exercise bout. Acute exercise is shown to have no effect on uncoupling protein 3 protein expression in plantaris, soleus or extensor digitorum longus muscle homogenates. Furthermore, we present novel results illustrating that endurance exercise (17 weeks) results in no significant changes in uncoupling protein 3 protein expression in whole muscle homogenates gastrocnemius, soleius, plantaris or extensor digitorum longus.
Author: Brennan, Clare M.
Qualification name:Doctor of Philosophy (Ph.D.)
Publisher:Trinity College (Dublin, Ireland). School of Biochemistry and Immunology
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Type of material:thesis
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