Show simple item record

dc.contributor.authorPidgeon, Grahamen
dc.contributor.authorLysaght, Joanneen
dc.contributor.authorO'Byrne, Kenen
dc.contributor.authorGately, Kathyen
dc.date.accessioned2017-05-25T11:24:07Z
dc.date.available2017-05-25T11:24:07Z
dc.date.issued2016en
dc.date.submitted2016en
dc.identifier.citationCathcart M.-C, Useckaite Z, Drakeford C, Semik V, Lysaght J, Gately K, O'Byrne K.J, Pidgeon G.P, Anti-cancer effects of baicalein in non-small cell lung cancer in-vitro and in-vivo, BMC Cancer, 16, 1, 2016, 707-en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/80227
dc.descriptionPUBLISHEDen
dc.descriptionCited By :2 Export Date: 3 April 2017en
dc.description.abstractBackground Baicalein is a widely used Chinese herbal medicine derived from Scutellaria baicalenesis, which has been traditionally used as anti-inflammatory and anti-cancer therapy. In this study we examined the anti-tumour pathways activated following baicalein treatment in non-small cell lung cancer (NSCLC), both in-vitro and in-vivo. Methods The effect of baicalein treatment on H-460 cells in-vitro was assessed using both BrdU assay (cell proliferation) and High Content Screening (multi-parameter apoptosis assay). A xenograft nude mouse model was subsequently established using these cells and the effect of baicalein on tumour growth and survival assessed in-vivo. Tumours were harvested from these mice and histological tissue analysis carried out. VEGF, 12-lipoxygenase and microvessel density (CD-31) were assessed by immunohistochemistry (IHC), while H and E staining was carried out to assess mitotic index. Gene expression profiling was carried out on corresponding RNA samples using Human Cancer Pathway Finder Arrays and qRT-PCR, with further gene expression analysis carried out using qRT-PCR. Results Baicalein significantly decreased lung cancer proliferation in H-460 cells in a dose dependent manner. At the functional level, a dose-dependent induction in apoptosis associated with decreased cellular f-actin content, an increase in nuclear condensation and an increase in mitochondrial mass potential was observed. Orthotopic treatment of experimental H-460 tumours in athymic nude mice with baicalein significantly (p < 0.05) reduced tumour growth and prolonged survival. Histological analysis of resulting tumour xenografts demonstrated reduced expression of both 12-lipoxygenase and VEGF proteins in baicalein-treated tumours, relative to untreated. A significant (p < 0.01) reduction in both mitotic index and micro-vessel density was observed following baicalein treatment. Gene expression profiling revealed a reduction (p < 0.01) in both VEGF and FGFR-2 following baicalein treatment, with a corresponding increase (p < 0.001) in RB-1. Conclusion This study is the first to demonstrate efficacy of baicalein both in-vitro and in-vivo in NSCLC. These effects may be mediated in part through a reduction in both cell cycle progression and angiogenesis. At the molecular level, alterations in expression of VEGF, FGFR-2, and RB-1 have been implicated, suggesting a molecular mechanism underlying this in-vivo effecten
dc.description.sponsorshipThis work was supported by a project grant from the Irish Cancer Society (grant number: CRI05PID; registered charity number: CHY5863) and also by a research fellowship from the Irish Cancer Society (grant number: CRI07OBY).en
dc.format.extent707en
dc.language.isoenen
dc.relation.ispartofseriesBMC Canceren
dc.relation.ispartofseries16en
dc.relation.ispartofseries1en
dc.rightsYen
dc.subjectScutellaria baicalenesisen
dc.subject.lcshScutellaria baicalenesisen
dc.titleAnti-cancer effects of baicalein in non-small cell lung cancer in-vitro and in-vivoen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/pidgeongen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/jlysaghten
dc.identifier.peoplefinderurlhttp://people.tcd.ie/gatelyken
dc.identifier.peoplefinderurlhttp://people.tcd.ie/obyrnekeen
dc.identifier.rssinternalid156519en
dc.identifier.doihttp://dx.doi.org/10.1186/s12885-016-2740-0en
dc.rights.ecaccessrightsopenAccess
dc.subject.TCDThemeCanceren
dc.identifier.rssurihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84984798022&doi=10.1186%2fs12885-016-2740-0&partnerID=40&md5=6549ae950049697cb07905337403903den
dc.identifier.orcid_id0000-0003-3363-3763en
dc.status.accessibleNen


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record