| dc.contributor.author | RAMASWAMI, MANI | en |
| dc.date.accessioned | 2017-05-23T10:49:22Z | |
| dc.date.available | 2017-05-23T10:49:22Z | |
| dc.date.issued | 2016 | en |
| dc.date.submitted | 2016 | en |
| dc.identifier.citation | Sarov M, Barz C, Jambor H, Hein M.Y, Schmied C, Suchold D, Stender B, Janosch S, Vinay Vikas K.J, Krishnan R.T, Krishnamoorthy A, Ferreira I.R.S, Ejsmont R.K, Finkl K, Hasse S, Kÿmpfer P, Plewka N, Vinis E, Schloissnig S, Knust E, Hartenstein V, Mann M, Ramaswami M, VijayRaghavan K, Tomancak P, Schnorrer F, A genome-wide resource for the analysis of protein localisation in Drosophila, eLife, 5, FEBRUARY, 2016, e12068- | en |
| dc.identifier.other | Y | en |
| dc.identifier.uri | http://hdl.handle.net/2262/80158 | |
| dc.description | PUBLISHED | en |
| dc.description.abstract | The Drosophila genome contains >13000 protein-coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here, we present a genome-wide fosmid library of 10000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins, we created transgenic lines, and for a total of 207 lines, we assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we visualised many proteins at endogenous expression levels and found a large fraction of them localising to subcellular compartments. By applying genetic complementation tests, we estimate that about two-thirds of the tagged proteins are functional. Moreover, these tagged proteins enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will boost systematic analysis of protein expression and localisation in various cellular and developmental contexts. | en |
| dc.description.sponsorship | Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study. We are grateful to Belinda Bullard, Anne Ephrussi, Judith Saide, Jürgen Knoblich, Stefan Baumgartner, Kathleen Clark, Jim Vigoreaux and the DSHB for generously sharing antibodies and fly lines. We thank Franziska Friedrich for drawing the ovariole scheme used for Figure 3A. We thank Andreas Dahl from the Deep Sequencing Group at CRTD/BIOTECH, Dresden for the NGS library preparation and sequencing. We also thank the light microscopy facility and the computer department for assistance with imaging and data processing. We are grateful to Sandra Lemke, Aynur Kaya-Copur and Xu Zhang for help with the genetic rescue experiments and to Cornelia Schönbauer, Beatrice Laudenbach and Caroline Sonsteby for assistance during some of the adult and pupal imaging experiments. We thank the entire Schnorrer lab for helpful comments on this manuscript. We are particularly grateful to Reinhard Fässler and Herbert Jäckle for continuous support of this work. This work was funded by the EMBO Young Investigator Program (FS), the European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013)/ERC Grant 310939 (FS), the European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013)/ERC Grant 260746 (PT), European Commission GENCODYS (PT and HJ), Human Frontier Science Program (HFSP) RGY0093/2012 (PT and CS) and the Max Planck Society (PT, FS, MS, MM and EK). | en |
| dc.format.extent | e12068 | en |
| dc.relation.ispartofseries | eLife | en |
| dc.relation.ispartofseries | 5 | en |
| dc.relation.ispartofseries | FEBRUARY | en |
| dc.rights | Y | en |
| dc.title | A genome-wide resource for the analysis of protein localisation in Drosophila | en |
| dc.type | Journal Article | en |
| dc.type.supercollection | scholarly_publications | en |
| dc.type.supercollection | refereed_publications | en |
| dc.identifier.peoplefinderurl | http://people.tcd.ie/ramaswam | en |
| dc.identifier.rssinternalid | 168920 | en |
| dc.identifier.doi | http://dx.doi.org/10.7554/eLife.12068 | en |
| dc.rights.ecaccessrights | openAccess | |
| dc.identifier.rssuri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84961266534&doi=10.7554%2feLife.12068&partnerID=40&md5=a3a64bf3551a9d2fef4fbc4203e85a05 | en |
