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dc.contributor.advisorDavey, Gavin
dc.contributor.authorWilliams, Emma
dc.date.accessioned2017-02-09T15:05:40Z
dc.date.available2017-02-09T15:05:40Z
dc.date.issued2011
dc.identifier.citationEmma Williams, 'Derivation of dopaminergic neurons from embryonic and induced pluripotent stem cells', [thesis], Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2011, pp 276
dc.identifier.otherTHESIS 9118
dc.identifier.urihttp://hdl.handle.net/2262/79398
dc.description.abstractDopamine (DA) neurons have several fundamental functions in the brain, dysfunction of which has been implicated in neurological and psychiatric disorders, including Parkinson’s disease (PD) and schizophrenia. Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) may provide an unlimited source of DA neurons for in vitro studies as they can be propagated indefinitely in culture in an undifferentiated state while retaining pluripotency. Interest has also focused on the use of DA neurons derived from these cells for regenerative medicine in PD. However, the potential of ESCs and iPSCs is greatly hindered by the heterogonous nature of cultures even after directed differentiation, with multiple cell types often of more than one germ layer being present. This may be partly or wholly overcome by expression of a marker, such as enhanced green fluorescent protein (EGEP), from a cell specific promoter, thus allowing both the selection of a particular cell type from a mixed cell population by fluorescent activated cell sorting (FACS) and differentiation to be followed in real time in live cells.
dc.format1 volume
dc.language.isoen
dc.publisherTrinity College (Dublin, Ireland). School of Biochemistry and Immunology
dc.relation.isversionofhttp://stella.catalogue.tcd.ie/iii/encore/record/C__Rb14632003
dc.subjectBiochemistry, Ph.D.
dc.subjectPh.D. Trinity College Dublin
dc.titleDerivation of dopaminergic neurons from embryonic and induced pluripotent stem cells
dc.typethesis
dc.type.supercollectionthesis_dissertations
dc.type.supercollectionrefereed_publications
dc.type.qualificationlevelDoctoral
dc.type.qualificationnameDoctor of Philosophy (Ph.D.)
dc.rights.ecaccessrightsopenAccess
dc.format.extentpaginationpp 276
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