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dc.contributor.advisorBond, Ursula
dc.contributor.authorKavanagh, Joanne Denise
dc.date.accessioned2017-01-19T15:30:37Z
dc.date.available2017-01-19T15:30:37Z
dc.date.issued2012
dc.identifier.citationJoanne Denise Kavanagh, 'Antimicrobial peptide preventing beer spoilage', [thesis], Trinity College (Dublin, Ireland). Department of Microbiology, 2012, pp 294
dc.identifier.otherTHESIS 9707
dc.identifier.urihttp://hdl.handle.net/2262/79064
dc.description.abstractBeer spoilage micro-organisms (BSMs) are a common threat to Master Brewers worldwide. Numerous studies have been carried out to date to overcome this problem, yet the most common preventative measure is the addition of chemical preservatives to the beverage. The aim of the research outlined in this thesis was to generate a series of Saccharomyces yeast strains with antimicrobial ability to minimize or eliminate beer spoilage micro-organisms without affecting the fermentation process. The human defensin, HβD-3, was chosen in this study due to its broad antimicrobial spectrum and nutraceutic potential. HβD-3 was cloned into Saccharomyces cerevisiae and Saccharomyces pastorianus on low and high copy number plasmids, under the control of a variety of gene promoters such as the inducible galactokinase (GAL1) promoter, the inducible a-glucosidase (AGT1) promoter, the constitutive translational elongation factor EF-1 alpha (TEF1) promoter and the constitutive phosphoglycerate kinase (PGKI) promoter. HβD-3 was also integrated directly into the genome of S. pastorianus.
dc.format1 volume
dc.language.isoen
dc.publisherTrinity College (Dublin, Ireland). Department of Microbiology
dc.relation.isversionofhttp://stella.catalogue.tcd.ie/iii/encore/record/C__Rb15152601
dc.subjectMicrobiology, Ph.D.
dc.subjectPh.D. Trinity College Dublin
dc.titleAntimicrobial peptide preventing beer spoilage
dc.typethesis
dc.type.supercollectionthesis_dissertations
dc.type.supercollectionrefereed_publications
dc.type.qualificationlevelDoctoral
dc.type.qualificationnameDoctor of Philosophy (Ph.D.)
dc.rights.ecaccessrightsopenAccess
dc.format.extentpaginationpp 294
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