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dc.contributor.authorGEOGHEGAN, JOANen
dc.contributor.authorFOSTER, TIMOTHYen
dc.date.accessioned2017-01-17T11:38:25Z
dc.date.available2017-01-17T11:38:25Z
dc.date.issued2015en
dc.date.submitted2015en
dc.identifier.citationKwiecinski J, Peetermans M, Liesenborghs L, Na M, Björnsdottir H, Zhu X, Jacobsson G, Johansson BR, Geoghegan JA, Foster TJ, Josefsson E, Bylund J, Verhamme P, Jin T, Staphylokinase Control of Staphylococcus aureus Biofilm Formation and Detachment Through Host Plasminogen Activation., The Journal of infectious diseases, 2015, 139-148en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/78769
dc.descriptionPUBLISHEDen
dc.description.abstractStaphylococcus aureus biofilms, a leading cause of persistent infections, are highly resistant to immune defenses and antimicrobial therapies. In the present study, we investigated the contribution of fibrin and staphylokinase (Sak) to biofilm formation. In both clinical S. aureus isolates and laboratory strains, high Sak-producing strains formed less biofilm than strains that lacked Sak, suggesting that Sak prevents biofilm formation. In addition, Sak induced detachment of mature biofilms. This effect depended on plasminogen activation by Sak. Host-derived fibrin, the main substrate cleaved by Sak-activated plasminogen, was a major component of biofilm matrix, and dissolution of this fibrin scaffold greatly increased susceptibility of biofilms to antibiotics and neutrophil phagocytosis. Sak also attenuated biofilm-associated catheter infections in mouse models. In conclusion, our results reveal a novel role for Sak-induced plasminogen activation that prevents S. aureus biofilm formation and induces detachment of existing biofilms through proteolytic cleavage of biofilm matrix components.en
dc.description.sponsorshipThis work was supported by the Swedish Medical Research Council, the Göteborg Medical Society, the Swedish Medical Soci- ety, the Rune and Ulla Amlövs Foundation, the Tore Nilsons Foundation, the Wilhelm and Martina Lundgren Foundation, the Stiftelsen Clas Gro- schinskys Minnesfond, the Adlerbertska Foundation, King Gustaf the 5th Memorial Foundation, the Royal Society of Arts and Sciences in Gothen- burg, the Scandinavian Society forAntimicrobial Chemotherapy Foundation, the Swedish Heart-Lung Foundation, the University of Gothenburg, and the Research Foundation Flanders (FWO-Vlaanderen; grants 11I0113N and 11S5416N)en
dc.format.extent139-148en
dc.relation.ispartofseriesThe Journal of infectious diseasesen
dc.rightsYen
dc.subjectStaphylococcus aureus biofilmsen
dc.subject.lcshStaphylococcus aureus biofilmsen
dc.titleStaphylokinase Control of Staphylococcus aureus Biofilm Formation and Detachment Through Host Plasminogen Activation.en
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/tfosteren
dc.identifier.peoplefinderurlhttp://people.tcd.ie/geoghejoen
dc.identifier.rssinternalid106762en
dc.identifier.doihttp://dx.doi.org/10.1093/infdis/jiv360en
dc.rights.ecaccessrightsopenAccess
dc.subject.TCDThemeGenes & Societyen
dc.subject.TCDThemeImmunology, Inflammation & Infectionen
dc.subject.TCDThemeNext Generation Medical Devicesen


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