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dc.contributor.authorMOVIA, DANIAen
dc.contributor.authorPRINA MELLO, ADRIELEen
dc.date.accessioned2017-01-13T14:30:16Z
dc.date.available2017-01-13T14:30:16Z
dc.date.issued2014en
dc.date.submitted2014en
dc.identifier.citationCatherine A Schütz, Davide Staedler, Kieran Crosbie-Staunton, Dania Movia, Catherine Chapuis Bernasconi, Blanka Halamoda Kenzaoui, Adriele Prina-Mello, Lucienne Juillerat-Jeannere, Differential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticles, International Journal of Nanomedicine, 9, 2014, 3481 - 3498en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/78737
dc.descriptionPUBLISHEDen
dc.description.abstractTherapeutic engineered nanoparticles (NPs), including ultrasmall superparamagnetic iron oxide (USPIO) NPs, may accumulate in the lower digestive tract following ingestion or injection. In order to evaluate the reaction of human colon cells to USPIO NPs, the effects of non-stabilized USPIO NPs (NS-USPIO NPs), oleic-acid-stabilized USPIO NPs (OA-USPIO NPs), and free oleic acid (OA) were compared in human HT29 and CaCo2 colon epithelial cancer cells. First the biophysical characteristics of NS-USPIO NPs and OA-USPIO NPs in water, in cell culture medium supplemented with fetal calf serum, and in cell culture medium preconditioned by HT29 and CaCo2 cells were determined. Then, stress responses of the cells were evaluated following exposure to NS-USPIO NPs, OA-USPIO NPs, and free OA. No modification of the cytoskeletal actin network was observed. Cell response to stress, including markers of apoptosis and DNA repair, oxidative stress and degradative/autophagic stress, induction of heat shock protein, or lipid metabolism was determined in cells exposed to the two NPs. Induction of an autophagic response was observed in the two cell lines for both NPs but not free OA, while the other stress responses were cell- and NP-specific. The formation of lipid vacuoles/droplets was demonstrated in HT29 and CaCo2 cells exposed to OA-USPIO NPs but not to NS-USPIO NPs, and to a much lower level in cells exposed to equimolar concentrations of free OA. Therefore, the induction of lipid vacuoles in colon cells exposed to OA utilized as a stabilizer for USPIO NPs is higly amplified compared to free OA, and is not observed in the absence of this lipid in NS-USPIO NPs.en
dc.description.sponsorshipThe authors want to thank the TCD CRANN Advanced Microscopy Laboratory, and in particular Dr Valerie Gerard, for the preparation of the TEM images of the received NPs and Seher Güney-Ayra for technical assistance. This work was partially supported by the European Commission 7th Framework Projects NanoImpactNet (NMP4-CA-2008-21853), NanoTEST (HEALTH-2007–201335), NAMDIATREAM (NMP4-LA-2010-246479), MULTIFUN (NMP4-LA-2011-262943), and NANoREG (NMP6-LA-2013-310584).en
dc.format.extent3481en
dc.format.extent3498en
dc.language.isoenen
dc.relation.ispartofseriesInternational Journal of Nanomedicineen
dc.relation.ispartofseries9en
dc.rightsYen
dc.subjectApoptosis; Caco-2 Cells; Heat-Shock Proteins; HT29 Cells; Humans; Lipids; Magnetite Nanoparticles; Oleic Acid; Particle Size; Stress, Physiological; Vacuolesen
dc.subject.lcshApoptosis; Caco-2 Cells; Heat-Shock Proteins; HT29 Cells; Humans; Lipids; Magnetite Nanoparticles; Oleic Acid; Particle Size; Stress, Physiological; Vacuolesen
dc.titleDifferential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticlesen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/dmoviaen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/prinameaen
dc.identifier.rssinternalid98960en
dc.identifier.doihttp://dx.doi.org/10.2147/IJN.S65082en
dc.rights.ecaccessrightsopenAccess
dc.relation.sourceDOIen
dc.subject.TCDThemeCanceren
dc.subject.TCDThemeNanoscience & Materialsen
dc.subject.TCDTagCANCER TREATMENTen
dc.subject.TCDTagNanoMedicineen
dc.subject.TCDTagNanosafetyen
dc.subject.TCDTagTranslational Nanomedicineen
dc.relation.sourceurihttp://dx.doi.org/10.2147/IJN.S65082en
dc.identifier.orcid_id0000-0001-6412-8132en


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