A clinical pharmacokinetic evaluation of Amphotericin B Lipid Complex in critically ill patients
Citation:Maeve E. Malone, 'A clinical pharmacokinetic evaluation of Amphotericin B Lipid Complex in critically ill patients', [thesis], Trinity College (Dublin, Ireland). School of Pharmacy & Pharmaceutical Sciences, 2012, pp 270
Malone TCD THESIS 10146 A clinical.pdf (PDF) 148.8Mb
Assay methods were successfully developed using High Performance Liquid Chromatography/Ultraviolet detection (HPLC/UV) in order to quantify amphotericin B (AMB) in patient plasma, ultrafiltrate (UF) and tracheal aspirate (TA) samples. Plasma and TA samples underwent solvent extraction and UF samples underwent solid phase extraction, prior to injection onto the HPLC column. AMB was detected at 405 nm. Plasma, UF and TA samples, collected from patients treated with ABLC, were shown to be stable when stored at -20°C until analysis. On day 1 of ABLC treatment, plasma samples were taken immediately before intravenous administration of ABLC and 1, 2, 3, 6, 12 and 24 hours after the start of the ABLC infusion. On subsequent days, pre- and post-ABLC infusion plasma samples were collected. Upon commencing or discontinuing Continuous Veno Venous Haemodiafiltration (CWHDF) during ABLC treatment, day 1 plasma sampling was repeated. On the cessation of ABLC treatment, plasma samples were collected at 24-hourly intervals for up to 96 hours, where possible. Patient UF was collected from each CWHDF UF bag used in a 24 hour dosage interval from the start of one ABLC infusion until the next infusion. During ABLC treatment and when clinically indicated, patient TA samples were taken up to a maximum of 5 TA samples in a 24 hour dosage interval.
Author: Malone, Maeve E.
Corrigan, Owen I.
Publisher:Trinity College (Dublin, Ireland). School of Pharmacy & Pharmaceutical Sciences
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Type of material:thesis
Availability:Full text available