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dc.contributor.authorKELLY, DANIELen
dc.contributor.authorBUCKLEY, CONORen
dc.contributor.authorVINARDELL, TATIANAen
dc.date.accessioned2016-09-16T11:04:29Z
dc.date.available2016-09-16T11:04:29Z
dc.date.created2015en
dc.date.issued2015en
dc.date.submitted2015en
dc.identifier.citationMesallati T, Sheehy E.J, Vinardell T, Buckley C.T, Kelly D.J, Tissue engineering scaled-up, anatomically shaped osteochondral constructs for joint resurfacing, European Cells and Materials, 30, 2015, 163 - 186en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/77352
dc.descriptionPUBLISHEDen
dc.descriptionExport Date: 26 November 2015en
dc.description.abstractArthroplasty is currently the only surgical procedure available to restore joint function following articular cartilage and bone degeneration associated with diseases such as osteoarthritis (OA). A potential alternative to this procedure would be to tissue-engineer a biological implant and use it to replace the entire diseased joint. The objective of this study was therefore to tissue-engineer a scaled-up, anatomically shaped, osteochondral construct suitable for partial or total resurfacing of a diseased joint. To this end it was first demonstrated that a bone marrow derived mesenchymal stem cell seeded alginate hydrogel could support endochondral bone formation in vivo within the osseous component of an osteochondral construct, and furthermore, that a phenotypically stable layer of articular cartilage could be engineered over this bony tissue using a co-culture of chondrocytes and mesenchymal stem cells. Co-culture was found to enhance the in vitro development of the chondral phase of the engineered graft and to dramatically reduce its mineralisation in vivo. In the final part of the study, tissue-engineered grafts (~ 2 cm diameter) mimicking the geometry of medial femorotibial joint prostheses were generated using laser scanning and rapid prototyped moulds. After 8 weeks in vivo, a layer of cartilage remained on the surface of these scaled-up engineered implants, with evidence of mineralisation and bone development in the underlying osseous region of the graft. These findings open up the possibility of a tissueengineered treatment option for diseases such as OAen
dc.description.sponsorshipFunding was provided by the Irish Research Council for Science, Engineering and Technology (G30403), the SFI President of Ireland Young Researcher Award (08/Y15/ B1336), and a European Research Council Starter Grant (StemRepair-Project number: 258463). We would like to acknowledge the contribution of Simon McCoy in creating the presented ABS moulds through rapid prototyping techniques.en
dc.format.extent163en
dc.format.extent186en
dc.relation.ispartofseriesEuropean Cells and Materialsen
dc.relation.ispartofseries30en
dc.rightsYen
dc.subjectalginic aciden
dc.subject.lcshalginic aciden
dc.titleTissue engineering scaled-up, anatomically shaped osteochondral constructs for joint resurfacingen
dc.typeJournal Articleen
dc.contributor.sponsorIrish Research Council for Science and Engineering Technology (IRCSET)en
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/kellyd9en
dc.identifier.peoplefinderurlhttp://people.tcd.ie/vinardten
dc.identifier.peoplefinderurlhttp://people.tcd.ie/cbuckleen
dc.identifier.rssinternalid108061en
dc.rights.ecaccessrightsopenAccess
dc.contributor.sponsorGrantNumberG30403en
dc.contributor.sponsorGrantNumber08/Y15/ B1336en
dc.identifier.rssurihttp://www.ecmjournal.org/journal/papers/vol030/pdf/v030a12.pdfen
dc.identifier.rssurihttp://www.scopus.com/inward/record.url?eid=2-s2.0-84943649563&partnerID=40&md5=4bfe0379ab3c2605695b8d5e9d5b91e4en
dc.identifier.orcid_id0000-0003-4091-0992en


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