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dc.contributor.authorRADOMSKI, MAREK
dc.contributor.authorMEDINA MARTIN, CARLOS
dc.date.accessioned2016-02-25T12:09:48Z
dc.date.available2016-02-25T12:09:48Z
dc.date.issued2006
dc.date.submitted2006en
dc.identifier.citationMedina, C., Santana, A., Paz, M.C., Diaz, F., Farre, E., Salas, A., Radomski, M.W., Quintero, E., Matrix metalloproteinase-9 modulates intestinal injury in rats with transmural colitis., Journal of Leukocyte Biology, 79, 2006, 954, 962en
dc.identifier.otherY
dc.identifier.urihttp://hdl.handle.net/2262/75978
dc.description.abstractProteolysis and degradation of extracellular matrix by metalloproteinases (MMPs) may contribute to intestinal injury in inflammatory bowel disease. In the present study, we investigated the pathogenic role of gelatinases (MMP-9 and MMP-2) on transmural colonic injury in a rat model of chronic colitis, which was induced by intracolonic instillation of trinitrobenzene sulfonic acid (TNBS). The activity and expression of MMP-2 and MMP-9 were measured in colonic tissue and peripheral neutrophils by fluorescence, zymography, Western blot, or immunohistochemistry at different time-points. Furthermore, myeloperoxidase content in colonic homogenates was analyzed to evaluate inflammation. Finally, morphological changes were assessed following early or delayed administration of CGS-27023-A, a synthetic inhibitor of MMPs. We found that the induction of colitis led to a significant up-regulation in tissue gelatinase concentration, whereas no changes in collagenase activity were observed. In addition, up-regulation of pro-MMP-9, but not of pro-MMP-2, was found on Days 7 and 10 following the induction of colitis. Furthermore, transmural MMP-9 was detected by immunofluorescent staining in the inflamed tissue. Consistent with tissue samples, neutrophils from colitic rats showed a significantly increased activity of pro-MMP-9. Finally, early but not delayed treatment with CGS-27023-A attenuated colonic mucosal injury in rats with TNBS-induced colitis. In conclusion, up-regulation of MMP-9 in peripheral and colonic neutrophils modulates transmural colonic injury in rats with TNBS-induced colitis.en
dc.format.extent954en
dc.format.extent962en
dc.language.isoenen
dc.relation.ispartofseriesJournal of Leukocyte Biology;
dc.relation.ispartofseries79;
dc.rightsYen
dc.subjectinflammatory bowel diseaseen
dc.titleMatrix metalloproteinase-9 modulates intestinal injury in rats with transmural colitis.en
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/martinc2
dc.identifier.peoplefinderurlhttp://people.tcd.ie/radomskm
dc.identifier.rssinternalid54470
dc.identifier.doihttp://dx.doi.org/10.1189/jlb.1005544
dc.rights.ecaccessrightsopenAccess
dc.identifier.rssurihttp://www.jleukbio.org/cgi/reprint/79/5/954


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