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dc.contributor.authorRADOMSKI, MAREK
dc.contributor.authorSANTOS-MARTINEZ, MARIA
dc.contributor.authorMEDINA MARTIN, CARLOS
dc.date.accessioned2016-02-25T12:07:42Z
dc.date.available2016-02-25T12:07:42Z
dc.date.issued2006
dc.date.submitted2006en
dc.identifier.citationMedina, C., Jurasz, P., Santos-Martinez, M.J., Jeong, S.S., Mitzky, R., Chen, R., Radomski, M.W., Platelet aggregation induced by Caco-2 cells: regulation by matrix metalloproteinase-2 and adenosine diphosphate, Journal of Pharmacology and Experimental Therapeutics, 317, 2, 2006, 739, 745en
dc.identifier.otherY
dc.identifier.urihttp://hdl.handle.net/2262/75977
dc.descriptionPUBLISHEDen
dc.description.abstractFormation of tumor cell-platelet aggregates facilitates hematogenous metastases. However, molecular mechanisms implicated in tumor cell-induced platelet aggregation (TCIPA) in colon cancer are unclear. To investigate mechanisms of TCIPA induced by colon adenocarcinoma cells in vitro, human Caco-2 cells were used to study their interactions with platelets using aggregometry, zymography, phase-contrast microscopy, and flow cytometry. Caco-2-induced platelet aggregation in a concentration-dependent manner. This aggregation resulted in the release of matrix metalloproteinase (MMP)-2, as measured by zymography. In addition, flow cytometry showed a significant up-regulation of activated GpIIb/IIIa, total GpIIb/IIIa, GpIb, and P-selectin receptors on platelets. Inhibition of MMP-2 by phenantroline and degradation of ADP by APT102, respectively, resulted in inhibition of TCIPA. Furthermore, both phenantroline and APT102 significantly down-regulated the surface abundance of platelet receptors. Caco-2 cells aggregate platelets, at least in part, via releasing MMP-2 and ADP. Modulation of MMP-2 and ADP actions could have therapeutic value in colonic cancer.en
dc.format.extent739en
dc.format.extent745en
dc.language.isoenen
dc.relation.ispartofseriesJournal of Pharmacology and Experimental Therapeutics;
dc.relation.ispartofseries317;
dc.relation.ispartofseries2;
dc.rightsYen
dc.subjectCaco-2 Cellsen
dc.titlePlatelet aggregation induced by Caco-2 cells: regulation by matrix metalloproteinase-2 and adenosine diphosphateen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/martinc2
dc.identifier.peoplefinderurlhttp://people.tcd.ie/radomskm
dc.identifier.peoplefinderurlhttp://people.tcd.ie/santosmm
dc.identifier.rssinternalid54473
dc.identifier.doihttp://dx.doi.org/10.1124/jpet.105.098384
dc.rights.ecaccessrightsopenAccess
dc.identifier.rssurihttp://jpet.aspetjournals.org/cgi/reprint/317/2/739


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