dc.contributor.author | RADOMSKI, MAREK | en |
dc.contributor.author | SANTOS-MARTINEZ, MARIA | en |
dc.date.accessioned | 2016-01-07T15:45:15Z | |
dc.date.available | 2016-01-07T15:45:15Z | |
dc.date.issued | 2014 | en |
dc.date.submitted | 2014 | en |
dc.identifier.citation | Inkielewicz-Stepniak, I., Santos-Martinez, M.J., Medina, C., Radomski, M.W., Pharmacological and toxicological effects of co-exposure of human gingival fibroblasts to silver nanoparticles and sodium fluoride, International Journal of Nanomedicine, 9, 2014, 1677 - 1687 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/75511 | |
dc.description | PUBLISHED | en |
dc.description.abstract | BACKGROUND:
Silver nanoparticles (AgNPs) and fluoride (F) are pharmacological agents widely used in oral medicine and dental practice due to their anti-microbial/anti-cavity properties. However, risks associated with the co-exposure of local cells and tissues to these xenobiotics are not clear. Therefore, we have evaluated the effects of AgNPs and F co-exposure on human gingival fibroblast cells.
METHODS:
Human gingival fibroblast cells (CRL-2014) were exposed to AgNPs and/or F at different concentrations for up to 24 hours. Cellular uptake of AgNPs was examined by transmission electron microscopy. Downstream inflammatory effects and oxidative stress were measured by real-time quantitative polymerase chain reaction (PCR) and reactive oxygen species (ROS) generation. Cytotoxicity and apoptosis were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and real-time quantitative PCR and flow cytometry, respectively. Finally, the involvement of mitogen-activated protein kinases (MAPK) was studied using Western blot.
RESULTS:
We found that AgNPs penetrated the cell membrane and localized inside the mitochondria. Co-incubation experiments resulted in increased oxidative stress, inflammation, and apoptosis. In addition, we found that co-exposure to both xenobiotics phosphorylated MAPK, particularly p42/44 MAPK.
CONCLUSION:
A combined exposure of human fibroblasts to AgNPs and F results in increased cellular damage. Further studies are needed in order to evaluate pharmacological and potentially toxicological effects of AgNPs and F on oral health. | en |
dc.format.extent | 1677 | en |
dc.format.extent | 1687 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | International Journal of Nanomedicine | en |
dc.relation.ispartofseries | 9 | en |
dc.rights | Y | en |
dc.subject | oxidative stress | en |
dc.title | Pharmacological and toxicological effects of co-exposure of human gingival fibroblasts to silver nanoparticles and sodium fluoride | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/radomskm | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/santosmm | en |
dc.identifier.rssinternalid | 97690 | en |
dc.rights.ecaccessrights | openAccess | |
dc.subject.TCDTheme | Nanoscience & Materials | en |
dc.subject.TCDTag | Nanopharmacology | en |