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dc.contributor.authorCORR, SINEADen
dc.contributor.authorO'NEILL, LUKEen
dc.contributor.authorPALSSON, EVAen
dc.contributor.authorFALLON, PADRAICen
dc.date.accessioned2016-01-06T15:25:20Z
dc.date.available2016-01-06T15:25:20Z
dc.date.issued2014en
dc.date.submitted2014en
dc.identifier.citationCorr, S.C., Palsson-Mcdermott, E.M., Grishina, I., Barry, S.P., Aviello, G., Bernard, N.J., Casey, P.G., Ward, J.B.J., Keely, S.J., Dandekar, S., Fallon, P.G., O'Neill, L.A.J., MyD88 adaptor-like (Mal) functions in the epithelial barrier and contributes to intestinal integrity via protein kinase C, Mucosal Immunology, 7, 1, 2014, 57-67en
dc.identifier.issn19330219en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/75436
dc.descriptionPUBLISHEDen
dc.description.abstractThe prognosis of epithelial ovarian cancer is poor in part due to the high frequency of chemoresistance. Recent evidence points to the Toll-like receptor-4 (TLR4), and particularly its adaptor protein MyD88, as one potential mediator of this resistance. This study aims to provide further evidence that MyD88 positive cancer cells are clinically significant, stem-like and reproducibly detectable for the purposes of prognostic stratification. Expression of TLR4 and MyD88 was assessed immunohistochemically in 198 paraffin-embedded ovarian tissues and in an embryonal carcinoma model of cancer stemness. In parallel, expression of TLR4 and MyD88 mRNA and regulatory microRNAs (miR-21 and miR-146a) was assessed, as well as in a series of chemosensitive and resistant cancer cells lines. Functional analysis of the pathway was assessed in chemoresistant SKOV-3 ovarian cancer cells. TLR4 and MyD88 expression can be reproducibly assessed via immunohistochemistry using a semi-quantitative scoring system. TLR4 expression was present in all ovarian epithelium (normal and neoplastic), whereas MyD88 was restricted to neoplastic cells, independent of tumour grade and associated with reduced progression-free and overall survival, in an immunohistological specific subset of serous carcinomas, p<0.05. MiR-21 and miR-146a expression was significantly increased in MyD88 negative cancers (p<0.05), indicating their participation in regulation. Significant alterations in MyD88 mRNA expression were observed between chemosensitive and chemoresistant cells and tissue. Knockdown of TLR4 in SKOV-3 ovarian cells recovered chemosensitivity. Knockdown of MyD88 alone did not. MyD88 expression was down-regulated in differentiated embryonal carcinoma (NTera2) cells, supporting the MyD88+ cancer stem cell hypothesis. Our findings demonstrate that expression of MyD88 is associated with significantly reduced patient survival and altered microRNA levels and suggest an intact/functioning TLR4/MyD88 pathway is required for acquisition of the chemoresistant phenotype. Ex vivo manipulation of ovarian cancer stem cell (CSC) differentiation can decrease MyD88 expression, providing a potentially valuable CSC model for ovarian cancer.en
dc.format.extent57-67en
dc.language.isoenen
dc.relation.ispartofseriesMucosal Immunologyen
dc.relation.ispartofseries7en
dc.relation.ispartofseries1en
dc.rightsYen
dc.subjectOvarian canceren
dc.titleMyD88 adaptor-like (Mal) functions in the epithelial barrier and contributes to intestinal integrity via protein kinase Cen
dc.typeJournal Articleen
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/abautraen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/corrscen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/pfallonen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/laoneillen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/palssoneen
dc.identifier.rssinternalid90812en
dc.identifier.doihttp://dx.doi.org/10.1038/mi.2013.24en
dc.rights.ecaccessrightsopenAccess
dc.subject.TCDThemeImmunology, Inflammation & Infectionen
dc.subject.TCDTagBiomedical sciencesen


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