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dc.contributor.authorO'FARRELLY, CLIONAen
dc.date.accessioned2015-12-09T11:45:22Z
dc.date.available2015-12-09T11:45:22Z
dc.date.issued2008en
dc.date.submitted2008en
dc.identifier.citationDavies D, Meade KG, Herath S, Eckersall PD, Gonzalez D, White JO, Conlan RS, O'Farrelly C, Sheldon IM, Toll-like receptor and antimicrobial peptide expression in the bovine endometrium., Reproductive biology and endocrinology : RB&E, 6, 2008, 53en
dc.identifier.issn1477-7827en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/75171
dc.descriptionPUBLISHEDen
dc.description.abstractBACKGROUND: The endometrium is commonly infected with bacteria leading to severe disease of the uterus in cattle and humans. The endometrial epithelium is the first line of defence for this mucosal surface against bacteria and Toll-like receptors (TLRs) are a critical component of the innate immune system for detection of pathogen associated molecular patterns (PAMPs). Antimicrobial peptides, acute phase proteins and Mucin-1 (MUC-1) also provide non-specific defences against microbes on mucosal surfaces. The present study examined the expression of innate immune defences in the bovine endometrium and tested the hypothesis that endometrial epithelial cells express functional receptors of the TLR family and the non-specific effector molecules for defence against bacteria. METHODS: Bovine endometrial tissue and purified populations of primary epithelial and stromal cells were examined using RT-PCR for gene expression of TLRs, antimicrobial peptides and MUC-1. Functional responses were tested by evaluating the secretion of prostaglandin E(2) and acute phase proteins when cells were treated with bacterial PAMPs such as bacterial lipopolysaccharide (LPS) and lipoproteins. RESULTS: The endometrium expressed TLRs 1 to 10, whilst purified populations of epithelial cells expressed TLRs 1 to 7 and 9, and stromal cells expressed TLRs 1 to 4, 6, 7, 9 and 10. The TLRs appear to be functional as epithelial cells secreted prostaglandin E(2) in response to bacterial PAMPs. In addition, the epithelial cells expressed antimicrobial peptides, such as Tracheal and Lingual Antimicrobial Peptides (TAP and LAP) and MUC-1, which were upregulated when the cells were treated with LPS. However, the epithelial cells did not express appreciable amounts of the acute phase proteins haptoglobin or serum amyloid A. CONCLUSION: Epithelial cells have an essential role in the orchestration of innate immune defence of the bovine endometrium and are likely to be the key to prevention of endometrial infection with bacteria.en
dc.description.sponsorshipMartin Sheldon is a BBSRC Research Development Fellow (Grant No. BB/D02028X/1) and Darren Davies was a BBSRC CASE Student (BBS/S/N/2005/12367). The work was partly funded under a DEFRA LINK award by Pfizer Animal Health and BBSRC (F005121).en
dc.format.extent53en
dc.relation.ispartofseriesReproductive biology and endocrinology : RB&Een
dc.relation.ispartofseries6en
dc.rightsYen
dc.subjectbovine endometriumen
dc.subject.lcshbovine endometriumen
dc.titleToll-like receptor and antimicrobial peptide expression in the bovine endometrium.en
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/ofarreclen
dc.identifier.rssinternalid104661en
dc.identifier.doihttp://dx.doi.org/10.1186/1477-7827-6-53en
dc.rights.ecaccessrightsopenAccess


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