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dc.contributor.authorROBERTS, KIMen
dc.date.accessioned2015-06-17T15:16:55Z
dc.date.available2015-06-17T15:16:55Z
dc.date.issued2013en
dc.date.submitted2013en
dc.identifier.citationShelton H, Roberts KL, Molesti E, Temperton N, Barclay WS, Mutations in haemagglutinin that affect receptor binding and pH stability increase replication of a PR8 influenza virus with H5 HA in the upper respiratory tract of ferrets and may contribute to transmissibility., The Journal of general virology, 94, Pt 6, 2013, 1220-9en
dc.identifier.issn0022-1317en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/74171
dc.descriptionPUBLISHEDen
dc.description.abstractThe H5N1 influenza A viruses have circulated widely in the avian population for 10 years with only sporadic infection of humans observed and no sustained human to human transmission. Vaccination against potential pandemic strains is one strategy in planning for future influenza pandemics; however, the success of live attenuated vaccines for H5N1 has been limited, due to poor replication in the human upper respiratory tract (URT). Mutations that increase the ability of H5N1 viruses to replicate in the URT will aid immunogenicity of these vaccines and provide information about humanizing adaptations in H5N1 strains that may signal transmissibility. As well as mediating receptor interactions, the haemagglutinin (HA) protein of influenza facilitates fusion of the viral membrane and genome entry into the host cell; this process is pH dependent. We have shown in this study that the pH at which a panel of avian influenza HA proteins, including H5, mediate fusion is higher than that for human influenza HA proteins, and that mutations in the H5 HA can reduce the pH of fusion. Coupled with receptor switching mutations, increasing the pH stability of the H5 HA resulted in increased viral shedding of H5N1 from the nasal cavity of ferrets and contact transmission to a co-housed animal. Ferret serum antibodies induced by infection with any of the mutated H5 HA viruses neutralized HA pseudotyped lentiviruses bearing homologous or heterologous H5 HAs, suggesting that this strategy to increase nasal replication of a vaccine virus would not compromise vaccine efficacy.en
dc.description.sponsorshipWe thank Dr Diane Major, NIBSC, South Mimms, UK, for providing ferret sera against A/Vietnam/1194/04, RG14 and RG23 viruses, and Dr Jim Robertson for providing RG14 and RG23 viruses. We thank the Central Biological Services team at St Mary’s Campus, Imperial College London, for their support with the ferret studies, and Anton de Paiva and Ian Hackford for health and safety advice. This work was funded by MRC project grant G06000504 and Wellcome Trust programme grant 087039/Z/08/Z to W. S. B.en
dc.format.extent1220-9en
dc.language.isoenen
dc.relation.ispartofseriesThe Journal of general virologyen
dc.relation.ispartofseries94en
dc.relation.ispartofseriesPt 6en
dc.rightsYen
dc.subjectH5N1 influenza A virusesen
dc.subject.lcshH5N1 influenza A virusesen
dc.titleMutations in haemagglutinin that affect receptor binding and pH stability increase replication of a PR8 influenza virus with H5 HA in the upper respiratory tract of ferrets and may contribute to transmissibility.en
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/krobertsen
dc.identifier.rssinternalid93110en
dc.identifier.doihttp://dx.doi.org/10.1099/vir.0.050526-0en
dc.rights.ecaccessrightsopenAccess
dc.subject.TCDThemeImmunology, Inflammation & Infectionen
dc.subject.TCDTagINFLUENZAen
dc.subject.TCDTagMolecular Biologyen
dc.subject.TCDTagVirologyen
dc.identifier.rssurihttp://vir.sgmjournals.org/content/94/Pt_6/1220.longen


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