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dc.contributor.authorROBERTS, KIMen
dc.date.accessioned2015-06-17T15:16:50Z
dc.date.available2015-06-17T15:16:50Z
dc.date.issued2011en
dc.date.submitted2011en
dc.identifier.citationvan Doremalen N, Shelton H, Roberts KL, Jones IM, Pickles RJ, Thompson CI, Barclay WS, A single amino acid in the HA of pH1N1 2009 influenza virus affects cell tropism in human airway epithelium, but not transmission in ferrets., PloS one, 6, 10, 2011, e25755en
dc.identifier.issn1932-6203en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/74170
dc.descriptionPUBLISHEDen
dc.description.abstracthe first pandemic of the 21 st century, pandemic H1N1 2009 (pH1N1 2009), emerged from a swine-origin source. Although human infections with swine-origin influenza have been reported previously, none went on to cause a pandemic or indeed any sustained human transmission. In previous pandemics, specific residues in the receptor binding site of the haemagglutinin (HA) protein of influenza have been associated with the ability of the virus to transmit between humans. In the present study we investigated the effect of residue 227 in HA on cell tropism and transmission of pH1N1 2009. In pH1N1 2009 and recent seasonal H1N1 viruses this residue is glutamic acid, whereas in swine influenza it is alanine. Using human airway epithelium, we show a differential cell tropism of pH1N1 2009 compared to pH1N1 2009 E227A and swine influenza suggesting this residue may alter the sialic acid conformer binding preference of the HA. Furthermore, both pH1N1 2009 E227A and swine influenza multi-cycle viral growth was found to be attenuated in comparison to pH1N1 2009 in human airway epithelium. However this altered tropism and viral growth in human airway epithelium did not abrogate respiratory droplet transmission of pH1N1 2009 E227A in ferrets. Thus, acquisition of E at residue 227 was not solely responsible for the ability of pH1N1 2009 to transmit between humans.en
dc.description.sponsorshipThis work was supported by a PhD studentship awarded to NvD by the Health Protection Agency, and a Society for General Microbiology Presidents Fund award to NvD, and a Medical Research Council project grant G0600504 awarded to WSB. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscripten
dc.format.extente25755en
dc.language.isoenen
dc.relation.ispartofseriesPloS oneen
dc.relation.ispartofseries6en
dc.relation.ispartofseries10en
dc.rightsYen
dc.subjectswine-origin influenzaen
dc.subject.lcshswine-origin influenzaen
dc.titleA single amino acid in the HA of pH1N1 2009 influenza virus affects cell tropism in human airway epithelium, but not transmission in ferrets.en
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/krobertsen
dc.identifier.rssinternalid93114en
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0025755en
dc.rights.ecaccessrightsopenAccess
dc.subject.TCDThemeImmunology, Inflammation & Infectionen
dc.subject.TCDTagMicrobiologyen
dc.subject.TCDTagMolecular Biologyen
dc.subject.TCDTagVirologyen
dc.identifier.rssurihttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0025755en


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