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dc.contributor.authorKELLY, JOHNen
dc.date.accessioned2015-05-05T14:54:12Z
dc.date.available2015-05-05T14:54:12Z
dc.date.issued2014en
dc.date.submitted2014en
dc.identifier.citationHall,James P. J.P., Sánchez-Weatherby,Juan J., Alberti,Cora C., Quimper,Caroline Hurtado C.H., O'Sullivan,Kyra K., Brazier,John A. J.A., Winter,Graeme G., Sorensen,Thomas T., Kelly,John M. J.M., Cardin,David John D.J., Cardin,Christine J J C.J.J., Controlled dehydration of a ruthenium complex-DNA crystal induces reversible DNA kinking, Journal of the American Chemical Society, 136, 50, 2014, 17505-17512en
dc.identifier.issn00027863en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/73820
dc.descriptionPUBLISHEDen
dc.description.abstractHydration-dependent DNA deformation has been known since Rosalind Franklin recognised that the relative humidity of the sample had to be maintained to observe a single conformation in DNA fibre diffraction. We now report for the first time the crystal structure, at the atomic level, of a dehydrated form of a DNA duplex and demonstrate the reversible interconversion to the hydrated form at room temperature. This system, containing d(TCGGCGCCGA) in the presence of Λ-[Ru(TAP)2(dppz)]2+ (TAP = 1,4,5,8-tetraazaphenanthrene, dppz = dipyridophenazine), undergoes a partial transition from an A/B hybrid to the A-DNA conformation, at 84-79% relative humidity. This is accompanied by an increase in kink at the central step from 22° to 51°, with a large movement of the terminal bases forming the intercalation site. This transition is reversible on rehydration. Seven datasets, collected from one crystal at room temperature, show the consequences of dehydration at near-atomic resolution. This result highlights that crystals, traditionally thought of as static systems, are still dynamic and therefore can be the subject of further experimentation.en
dc.description.sponsorshipJ.P.H is funded by Diamond Light Source and the University of Reading. We acknowledge additional financial support from the Royal Society, the Royal Irish Academy and the Science Foundation Ireland (grant 06/RF/CHP035). This work was supported by BBSRC grant BB/K019279/1 awarded to C.J.C, J.A.B and T.S.en
dc.format.extent17505-17512en
dc.language.isoenen
dc.relation.ispartofseriesJournal of the American Chemical Societyen
dc.relation.ispartofseries136en
dc.relation.ispartofseries50en
dc.rightsYen
dc.subjectHydration-dependent DNA deformationen
dc.subject.lcshHydration-dependent DNA deformationen
dc.titleControlled dehydration of a ruthenium complex-DNA crystal induces reversible DNA kinkingen
dc.typeJournal Articleen
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/jmkellyen
dc.identifier.rssinternalid99656en
dc.identifier.doihttp://dx.doi.org/10.1021/ja508745xen
dc.rights.ecaccessrightsopenAccess
dc.contributor.sponsorGrantNumber06/RF/CHP035en


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